كتاب
In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls
| العنوان: | In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls |
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| المؤلفون: | Trinh, Hoang Khai, Verstraeten, Inge, Geelen, Danny |
| المساهمون: | Ristova, Daniela, Barbez, Elke |
| المصدر: | Root development : methods and protocols ; ISSN: 1064-3745 ; ISSN: 1940-6029 ; ISBN: 9781493977468 ; ISBN: 9781493992737 ; ISBN: 9781493977475 |
| بيانات النشر: | Humana |
| سنة النشر: | 2018 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, Etiolated seedlings, Hypocotyls, Adventitious roots |
| الوصف: | Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they are induced determine the physiological properties of emerged ARs. Hence, a standard method encompassing all types of AR does not exist. Here we describe a method for the induction and analysis of AR that emerge from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis and shows a determined developmental pattern which usually does not involve AR formation. However, the hypocotyl shows propensity to form de novo roots under specific circumstances such as removal of the root system, high humidity or flooding, or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer surrounding the vascular tissue of the central cylinder, which is reminiscent to the developmental program of lateral roots. Here we propose an easy protocol for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings. |
| نوع الوثيقة: | book part |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-4939-7746-8 978-1-4939-9273-7 978-1-4939-7747-5 1-4939-7746-6 1-4939-9273-2 1-4939-7747-4 |
| العلاقة: | https://biblio.ugent.be/publication/8556442Test; http://hdl.handle.net/1854/LU-8556442Test; http://dx.doi.org/10.1007/978-1-4939-7747-5_7Test; https://biblio.ugent.be/publication/8556442/file/8556496Test |
| DOI: | 10.1007/978-1-4939-7747-5_7 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-7747-5_7Test https://biblio.ugent.be/publication/8556442Test http://hdl.handle.net/1854/LU-8556442Test https://biblio.ugent.be/publication/8556442/file/8556496Test |
| حقوق: | No license (in copyright) ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.53A453AC |
| قاعدة البيانات: | BASE |
| ردمك: | 9781493977468 9781493992737 9781493977475 1493977466 1493992732 1493977474 |
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| DOI: | 10.1007/978-1-4939-7747-5_7 |