دورية أكاديمية
Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers
| العنوان: | Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers |
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| المؤلفون: | Qing Yang, Nicholas R Meyerson, Stephen K Clark, Camille L Paige, Will T Fattor, Alison R Gilchrist, Arturo Barbachano-Guerrero, Benjamin G Healy, Emma R Worden-Sapper, Sharon S Wu, Denise Muhlrad, Carolyn J Decker, Tassa K Saldi, Erika Lasda, Patrick Gonzales, Morgan R Fink, Kimngan L Tat, Cole R Hager, Jack C Davis, Christopher D Ozeroff, Gloria R Brisson, Matthew B McQueen, Leslie A Leinwand, Roy Parker, Sara L Sawyer |
| المصدر: | eLife, Vol 10 (2021) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | SARS-CoV-2, testing, university, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| العلاقة: | https://elifesciences.org/articles/65113Test; https://doaj.org/toc/2050-084XTest |
| DOI: | 10.7554/eLife.65113 |
| الوصول الحر: | https://doaj.org/article/d52bb77200174523b04c7043a7af4511Test |
| رقم الانضمام: | edsdoj.52bb77200174523b04c7043a7af4511 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.65113 |