التفاصيل البيبلوغرافية
| العنوان: |
Macrophage polarization in the maculae of age-related macular degeneration: A pilot study. |
| المؤلفون: |
Cao, Xiaoguang, Shen, Defen, Patel, Mrinali M., Tuo, Jingsheng, Johnson, T. Mark, Olsen, Timothy W., Chan, Chi-Chao |
| المصدر: |
Pathology International; Sep2011, Vol. 61 Issue 9, p528-535, 8p |
| مصطلحات موضوعية: |
MACROPHAGES, RETINAL degeneration, AGE factors in disease, POLYMERASE chain reaction, VASCULAR endothelial growth factors, CHEMOKINES, EYE aging |
| مستخلص: |
Macrophages can be polarized to exhibit either pro-inflammatory M1 or pro-angiogenic M2 phenotypes, but have high phenotypic plasticity. This pilot study investigated macrophage polarization in the macular retina and choroid of age-related macular degeneration (AMD) and non-AMD subjects, as well as in AMD choroidal neovascular membranes (CNVM). All specimens were evaluated for routine histopathology. Quantitative real-time polymerase chain reaction for representative M1 ( CXCL11) and M2 ( CCL22) transcripts were performed on macular choroidal trephines (MCT) of 19 AMD and nine non-AMD eye bank eyes, on the microdissected macular retinal cells from the archived slides of five geographic atrophic AMD, five exudative/neovascular AMD, and eight normal autopsied eyes, and on microdissected inflammatory cells from two surgically removed CNVM that did not respond to anti-vascular endothelial growth factor (VEGF) therapy. High M2-chemokine transcript and a low ratio of M1 to M2 chemokine transcript were found in aging non-AMD MCT. Advanced AMD maculae had a higher M1 to M2 chemokine transcript ratio compared to normal autopsied eyes. Macrophages in the two CNVM of patients unresponsive to anti-VEGF therapy were polarized toward either M1 or M2 phenotypes. The number of M2 macrophages was increased compared to M1 macrophages in normal aging eyes. A pathological shift of macrophage polarization may play a potential role in AMD pathogenesis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
