دورية أكاديمية
LRP10 interacts with SORL1 in the intracellular vesicle trafficking pathway in non-neuronal brain cells and localises to Lewy bodies in Parkinson’s disease and dementia with Lewy bodies
| العنوان: | LRP10 interacts with SORL1 in the intracellular vesicle trafficking pathway in non-neuronal brain cells and localises to Lewy bodies in Parkinson’s disease and dementia with Lewy bodies |
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| المؤلفون: | Grochowska M. M., Carreras Mascaro A., Boumeester V., Natale D., Breedveld G. J., Geut H., van Cappellen W. A., Boon A. J. W., Kievit A. J. A., Sammler E., Parchi P., Cortelli P., Alessi D. R., van de Berg W. D. J., Bonifati V., Mandemakers W. |
| المساهمون: | Grochowska M.M., Carreras Mascaro A., Boumeester V., Natale D., Breedveld G.J., Geut H., van Cappellen W.A., Boon A.J.W., Kievit A.J.A., Sammler E., Parchi P., Cortelli P., Alessi D.R., van de Berg W.D.J., Bonifati V., Mandemakers W. |
| سنة النشر: | 2021 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Astrocyte, Dementia with Lewy bodies (DLB), Lewy bodie, LRP10, Parkinson’s disease (PD), Vesicle trafficking, Adult, Aged, Brain, Genetic Variation, Human, Induced Pluripotent Stem Cell, LDL-Receptor Related Protein, Lewy Body Disease, Membrane Transport Protein, Middle Aged, Neurodegenerative Disease, Neuron, Parkinson Disease |
| الوصف: | Loss-of-function variants in the low-density lipoprotein receptor-related protein 10 (LRP10) gene have been associated with autosomal-dominant Parkinson’s disease (PD), PD dementia, and dementia with Lewy bodies (DLB). Moreover, LRP10 variants have been found in individuals diagnosed with progressive supranuclear palsy and amyotrophic lateral sclerosis. Despite this genetic evidence, little is known about the expression and function of LRP10 protein in the human brain under physiological or pathological conditions. To better understand how LRP10 variants lead to neurodegeneration, we first performed an in-depth characterisation of LRP10 expression in post-mortem brains and human-induced pluripotent stem cell (iPSC)-derived astrocytes and neurons from control subjects. In adult human brain, LRP10 is mainly expressed in astrocytes and neurovasculature but undetectable in neurons. Similarly, LRP10 is highly expressed in iPSC-derived astrocytes but cannot be observed in iPSC-derived neurons. In astrocytes, LRP10 is present at trans-Golgi network, plasma membrane, retromer, and early endosomes. Interestingly, LRP10 also partially co-localises and interacts with sortilin-related receptor 1 (SORL1). Furthermore, although LRP10 expression and localisation in the substantia nigra of most idiopathic PD and DLB patients and LRP10 variant carriers diagnosed with PD or DLB appeared unchanged compared to control subjects, significantly enlarged LRP10-positive vesicles were detected in a patient carrying the LRP10 p.Arg235Cys variant. Last, LRP10 was detected in Lewy bodies (LB) at late maturation stages in brains from idiopathic PD and DLB patients and in LRP10 variant carriers. In conclusion, high LRP10 expression in non-neuronal cells and undetectable levels in neurons of control subjects indicate that LRP10-mediated pathogenicity is initiated via cell non-autonomous mechanisms, potentially involving the interaction of LRP10 with SORL1 in vesicle trafficking pathways. Together with the specific pattern of LRP10 ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/33913039; info:eu-repo/semantics/altIdentifier/wos/WOS:000645075800001; volume:142; issue:1; firstpage:117; lastpage:137; numberofpages:21; journal:ACTA NEUROPATHOLOGICA; https://hdl.handle.net/11585/858952Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85105398816; https://link.springer.com/article/10.1007/s00401-021-02313-3Test |
| DOI: | 10.1007/s00401-021-02313-3 |
| الإتاحة: | https://doi.org/10.1007/s00401-021-02313-3Test https://hdl.handle.net/11585/858952Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C0A827B8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00401-021-02313-3 |
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