دورية أكاديمية
Interleukin 35 induced Th2 and Tregs bias under normal conditions in mice
| العنوان: | Interleukin 35 induced Th2 and Tregs bias under normal conditions in mice |
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| المؤلفون: | Xiaoning Zhang, Zhiqiang Zhang, Zhiqiang He, Mingyan Ju, Jiaci Li, Jinghua Yuan, Yaqing Jing, Keqiu Li, Yi Liu, Guang Li |
| المصدر: | PeerJ, Vol 6, p e5638 (2018) |
| بيانات النشر: | PeerJ Inc., 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | T-cell subsets, Interleukin 35, Helper T cells, Cytokines, Regulatory T cells, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Objective The benefits of IL-35 treatment have been verified in multiple animal models of diseases, while its influence on T cells immunity under normal condition still needs to be elucidated. The present study was designed to investigate the effects modulating IL-35 levels in vivo and in vitro on T cells, response and also the effects on T cells subsets in normal mice. Methods A plasmid pMSCV-IL-35-GFP carrying mouse linear IL-35 fragment with two subunits joint together was constructed and the heterodimer expression was confirmed. Normal mice were randomly divided into three groups and received an intravenous injection of PBS, pMSCV-GFP and pMSCV-IL-35-GFP respectively. After 72 h, spleen tissues and peripheral blood were harvested for following analysis. Meanwhile, splenic T cells were isolated and incubated with 10, 30, or 50 ng/mL recombinant IL-35 factor for 24 h with the addition of anti-CD3/CD28 in vitro. T-cell subsets were assessed by Fluorescence activated cell sorting (FACS) and related cytokines together with effector molecules were determined by real time PCR. Results Western blotting confirmed a 52 kDa band in the cell lysate of HEK 293T transducted with pMSCV-IL-35-GFP plasmid, indicating a successful expression of IL-35. Ebi3 and IL-12A, two subunits of IL-35, could be identified 72 h post DNA injection. IL-35 upregulation in vivo effectively inhibit CD4+ and CD8+ T cell proliferation and Th1 cytokine secretion. Effector molecules of CD8+ T cells were also remarkably suppressed. On the contrary, high level of IL-35 significantly induced CD4+ CD25+ Tregs and Th2 enhancement. The in vitro study provided similar results. Conclusion The results indicated Th1 and CD8+ T cell inhibition and Th2 and Tregs bias in the presence of IL-35 under a normal state which partly contributed to its therapeutic potential. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| العلاقة: | https://peerj.com/articles/5638.pdfTest; https://peerj.com/articles/5638Test/; https://doaj.org/toc/2167-8359Test |
| DOI: | 10.7717/peerj.5638 |
| الوصول الحر: | https://doaj.org/article/df95931928e446d1a260d4c4725e9d76Test |
| رقم الانضمام: | edsdoj.f95931928e446d1a260d4c4725e9d76 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.5638 |