دورية أكاديمية
Evolution of a Dominant Natural Isolate ofEscherichia coliin the Human Gut over the Course of a Year Suggests a Neutral Evolution with Reduced Effective Population Size
| العنوان: | Evolution of a Dominant Natural Isolate ofEscherichia coliin the Human Gut over the Course of a Year Suggests a Neutral Evolution with Reduced Effective Population Size |
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| المؤلفون: | Olivier Tenaillon, Olivier Clermont, Antoine Bridier-Nahmias, Erick Denamur, Mohamed Ghalayini, Adrien Launay, Mathilde Lescat |
| المصدر: | 10.1128/AEM.02377-17 ; 2783191953 ; oai:pubmedcentral.nih.gov:5835743 ; 29305507 ; 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 ; 10|issn___print::becac7b0e512df34fe0b73f71ab29e4e ; 10|openaire____::8ac8380272269217cb09a928c8caa993 ; 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a ; 10|opendoar____::eda80a3d5b344bc40f3bc04f65b7a357 ; 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c ; 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 ; 10|openaire____::55045bd2a65019fd8e6741a755395c8c ; openaire____::1256f046-bf1f-4afc-8b47-d0b147148b18 ; 10|openaire____::806360c771262b4d6770e7cdf04b5c5a |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Biotechnology, Food Science, Ecology, Applied Microbiology and Biotechnology, Genetics and Molecular Biology, envir, geo |
| الوصف: | In vitroandin vivoevolution experiments onEscherichia colirevealed several principles of bacterial adaptation. However, few data are available in the literature describing the behavior ofE. coliin its natural environment. We attempted here to study the evolution in the human gut of a commensal dominantE. coliclone, ED1a belonging to the B2 phylogroup, through a longitudinal genomic study. We sequenced 24 isolates sampled at three different time points within a healthy individual over almost a year. We computed a mutation rate of 6.90 × 10−7mutations per base per year of the chromosome forE. coliED1a in healthy human gut. We observed very limited genomic diversity and could not detect any evidence of selection, in contrast to what is observed in experimental evolution over a similar length of time. We therefore suggest that ED1a, being well adapted to the healthy human gut, evolves mostly neutrally with a low effective population size (Neof ≈500 to 1,700).IMPORTANCEIn this study, we follow the genomic fate of a dominant clone ofEscherichia coliin the human gut of a healthy individual over about a year. We could compute a low annual mutation rate that supports low diversity, and we could not retrieve any clear signature of selection. These observations support a neutral evolution ofE. coliin the human gut, compatible with a very limited effective population size that deviates drastically with the observations made previously in experimental evolution. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://aem.asm.org/content/aem/84/6/e02377-17.full.pdfTest; http://dx.doi.org/10.1128/aem.02377-17Test; https://aem.asm.org/content/84/6/e02377-17Test; https://www.ncbi.nlm.nih.gov/pubmed/29305507Test; http://europepmc.org/abstract/MED/29305507Test; https://academic.microsoft.com/#/detail/2783191953Test; https://europepmc.org/articles/PMC5835743Test/; https://dx.doi.org/10.1128/aem.02377-17Test |
| DOI: | 10.1128/AEM.02377-17 |
| DOI: | 10.1128/aem.02377-17 |
| الإتاحة: | https://doi.org/10.1128/aem.02377-17Test https://doi.org/10.1128/AEM.02377-17Test https://aem.asm.org/content/aem/84/6/e02377-17.full.pdfTest https://aem.asm.org/content/84/6/e02377-17Test https://www.ncbi.nlm.nih.gov/pubmed/29305507Test http://europepmc.org/abstract/MED/29305507Test https://academic.microsoft.com/#/detail/2783191953Test https://europepmc.org/articles/PMC5835743Test/ |
| حقوق: | undefined |
| رقم الانضمام: | edsbas.4602D3F7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/AEM.02377-17 |
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