Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery
| العنوان: | Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery |
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| المؤلفون: | Taiksang Nam, Seungsoo Jung, Young Ho Lee, Duck Sun Ahn, Jong-Eun Won |
| المصدر: | Yonsei Medical Journal |
| بيانات النشر: | Yonsei University College of Medicine, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Nitroprusside, medicine.medical_specialty, Contraction (grammar), Time Factors, Endothelium, Ca2+ regulation, chemistry.chemical_element, NG-nitro-L-arginine methyl ester, Calcium, Organ culture, Culture Media, Serum-Free, Organ Culture Techniques, Internal medicine, Medicine, Animals, Models, Statistical, biology, Dose-Response Relationship, Drug, business.industry, carotid artery, General Medicine, Anatomy, Acetylcholine, Nitric oxide synthase, Endocrinology, medicine.anatomical_structure, Carotid Arteries, NG-Nitroarginine Methyl Ester, chemistry, biology.protein, Original Article, Rabbits, medicine.symptom, business, Intracellular, medicine.drug, Muscle contraction, Muscle Contraction |
| الوصف: | The aim of the present study was to examine the functional changes that occur when a rabbit carotid artery is cultured in serum-free medium. In endothelium (EC)-intact arteries cultured under serum-free conditions, acetylcholine (ACh)-induced relaxation responses were partially, yet significantly, reduced when compared with freshly isolated arteries. After pretreatment with NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, application of ACh resulted in a significant contraction in organ cultured arteries. The amplitude of the ACh-induced contractions increased with the duration of culture. In EC-denuded arteries cultured under serum-free conditions, ACh induced responses similar to those in EC-intact arteries pretreated with L-NAME. Furthermore, ACh caused a significant increase in intracellular Ca2+ concentration ([Ca2+]i) in EC-denuded arteries cultured under serum-free condition for 7 days. There was little change in either [Ca2+]i or tension in freshly isolated carotid rings. There was no difference in sodium nitroprusside-induced relaxation responses between fresh and cultured arteries. These results suggest that prolonged culture of carotid arteries under serum- free conditions changes the functional properties of vascular reactivity in rabbit carotid arteries. |
| اللغة: | English |
| تدمد: | 1976-2437 0513-5796 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e686e13d5c79f65c34eba52933b18e72Test http://europepmc.org/articles/PMC2687636Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e686e13d5c79f65c34eba52933b18e72 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19762437 05135796 |
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