دورية أكاديمية
Metabolic Pathway Signatures Associated with Urinary Metabolite Biomarkers Differentiate Bladder Cancer Patients from Healthy Controls.
| العنوان: | Metabolic Pathway Signatures Associated with Urinary Metabolite Biomarkers Differentiate Bladder Cancer Patients from Healthy Controls. |
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| المساهمون: | College of Medicine, Dept. of Urology, Won Tae Kim, Seok Joong Yun, Chunri Yan, Pildu Jeong, Ye Hwan Kim, Il-Seok Lee, Ho-Won Kang, Sunghyouk Park, Sung-Kwon Moon, Yung-Hyun Choi, Young Deuk Choi, Isaac Yi Kim, Jayoung Kim, Wun-Jae Kim, Choi, Young Deuk |
| بيانات النشر: | Yonsei University Korea (South) |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Aged, Biomarkers/metabolism, Carcinoma, Transitional Cell/genetics, Transitional Cell/metabolism, Transitional Cell/pathology, Carnitine/analogs & derivatives, Carnitine/genetics, Carnitine/metabolism, Case-Control Studies, Female, Humans, Male, Metabolic Networks and Pathways/physiology, Middle Aged, RNA, Messenger/metabolism, Real-Time Polymerase Chain Reaction, Urinary Bladder Neoplasms/genetics, Urinary Bladder Neoplasms/metabolism, Urinary Bladder Neoplasms/pathology, Bladder cancer, diagnostic marker, gene expression, qRT-PCR, urine metabolites |
| الوصف: | PURPOSE: Our previous high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry study identified bladder cancer (BCA)-specific urine metabolites, including carnitine, acylcarnitines, and melatonin. The objective of the current study was to determine which metabolic pathways are perturbed in BCA, based on our previously identified urinary metabolome. MATERIALS AND METHODS: A total of 135 primary BCA samples and 26 control tissue samples from healthy volunteers were analyzed. The association between specific urinary metabolites and their related encoding genes was analyzed. RESULTS: Significant alterations in the carnitine-acylcarnitine and tryptophan metabolic pathways were detected in urine specimens from BCA patients compared to those of healthy controls. The expression of eight genes involved in the carnitine-acylcarnitine metabolic pathway (CPT1A, CPT1B, CPT1C, CPT2, SLC25A20, and CRAT) or tryptophan metabolism (TPH1 and IDO1) was assessed by RT-PCR in our BCA cohort (n=135). CPT1B, CPT1C, SLC25A20, CRAT, TPH1, and IOD1 were significantly downregulated in tumor tissues compared to normal bladder tissues (p<0.05 all) of patients with non-muscle invasive BCA, whereas CPT1B, CPT1C, CRAT, and TPH1 were downregulated in those with muscle invasive BCA (p<0.05), with no changes in IDO1 expression. CONCLUSION: Alterations in the expression of genes associated with the carnitine-acylcarnitine and tryptophan metabolic pathways, which were the most perturbed pathways in BCA, were determined. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 865~871 |
| اللغة: | English |
| تدمد: | 0513-5796 1976-2437 |
| العلاقة: | YONSEI MEDICAL JOURNAL; J02813; OAK-2016-02856; https://ir.ymlib.yonsei.ac.kr/handle/22282913/147012Test; T201601940; YONSEI MEDICAL JOURNAL, Vol.57(4) : 865-871, 2016 |
| DOI: | 10.3349/ymj.2016.57.4.865 |
| الإتاحة: | https://doi.org/10.3349/ymj.2016.57.4.865Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/147012Test |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ |
| رقم الانضمام: | edsbas.67D7E33C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 05135796 19762437 |
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| DOI: | 10.3349/ymj.2016.57.4.865 |