المؤلفون: Seulki Kim, A Reum Kim, Seungjin Lim, Su Jin Lee, Moonsuk Bae

المصدر: Asian Pacific Journal of Tropical Medicine, Vol 17, Iss 6, Pp 273-280 (2024)

مصطلحات موضوعية: scrub typhus, serological test, immunofluorescence assay, immunochromatography, rapid detecting test, Arctic medicine. Tropical medicine, RC955-962

الوصف: Objective: Serological tests are widely used for scrub typhus diagnosis; however, their limitations are evident. This study aims to assess their practical value in clinical settings. Methods: We analyzed the data of adult patients with suspected scrub typhus who visited a tertiary care hospital in the Republic of Korea from September to December from 2019 to 2021. The included patients had an acute fever and at least one of the following ten secondary findings: myalgia, skin rash, eschar, headache, thrombocytopenia, increased liver enzyme levels, lymphadenopathy, hepatomegaly, splenomegaly, and pleural effusion. The diagnoses were grouped as scrub typhus or other diseases by two infectious disease physicians. Results: Among 136 patients who met the eligibility criteria, 109 had scrub typhus and 27 had different diseases. Single and paired total antibodies using immunofluorescence assay (IFA), and total antibodies using immunochromatography-based rapid diagnostic testing (ICT) were measured in 98%, 22%, and 75% of all patients, respectively. Confirmation using paired samples for scrub typhus was established at a median of 11 [interquartile range (IQR) 10-16] days following the first visit. Among the 82 admitted patients, the median admission time was 9 (IQR 7-13) days. According to IFA, 58 (55%) patients with scrub typhus had total immunoglobulin titers ≥1:320, while 23 (85%) patients with other disease had titers < 1:320. Positive ICT results were observed in 64 (74%) patients with scrub typhus and 10 (67%) patients with other diseases showed negative ICT results. Conclusions: Serological testing for scrub typhus is currently insufficient for decision-making in clinical practice.

وصف الملف: electronic resource

العلاقة: https://journals.lww.com/10.4103/apjtm.apjtm_815_23Test; https://doaj.org/toc/2352-4146Test

الوصول الحر: https://doaj.org/article/8364eb17491b40a5a8a715c5092ff53bTest

المؤلفون: Devi Prasad Acharya, Aseem K Tiwari, Geet Aggarwal, Dinesh Arora, Ravi C Dara, Gunjan Bhardwaj, Gautam Kumar Gupta

المصدر: Global Journal of Transfusion Medicine, Vol 4, Iss 2, Pp 191-197 (2019)

مصطلحات موضوعية: automated treponema pallidum hemagglutination assay, fluorescent treponemal antibody-absorption assay, immunochromatographic assay, rapid plasma reagin, serological test for syphilis, syphilis, Diseases of the blood and blood-forming organs, RC633-647.5

الوصف: Introduction: Serological tests for syphilis contributed greatly to the detection of Treponema pallidum infection in blood donors and especially in those who were missed during the medical selection. Although several different treponemal assays have been evaluated in India, the debate that which treponemal test-formats would be suitable for a particular blood transfusion service is far from settled. Aims and Objectives: The study was undertaken with an objective of comparing conventional immunochromatographic assay (ICA) with new automated T. pallidum hemagglutination assay (a-TPHA) in over 10,000 consecutive blood donor samples with fluorescent treponemal antibody-absorption assay (FTA-Abs) as the gold standard to resolve the discordant samples. Materials and Methods: The study was conducted in the Department of Transfusion Medicine in a large tertiary care hospital in India. Consecutive blood donors from July 2014 to January 2015 were evaluated simultaneously for antitreponemal antibodies by solid-phase ICA (SD BIOLINE Syphilis 3.0, Alere Medical Pvt. Ltd., USA), a-TPHA (Immucor Diagnostics, USA) and FTA-Abs (Biocientifica SA, FTA-Abs, Argentina). Performances of both the assays were evaluated using statistical tools. Results: A total of 10,129 donor samples were evaluated. A total of 113 (1.1%) donor samples were reactive for syphilis; either on ICA or a-TPHA or both. Ninety-one of these 113 reactive samples were reactive on FTA-Abs, giving seroprevalance of syphilis as 0.9%. a-TPHA was superior to ICA in terms of sensitivity, specificity, positive predictive value, negative predictive value, and Youden's index. Conclusion: The new “a-TPHA” is a superior assay to ICA as the screening assay for syphilis in blood donors.

وصف الملف: electronic resource

العلاقة: http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2019;volume=4;issue=2;spage=191;epage=197;aulastTest=; https://doaj.org/toc/2468-8398Test; https://doaj.org/toc/2455-8893Test

الوصول الحر: https://doaj.org/article/4f4c61016dd6462ca5ca3d39fca5c2ebTest

المؤلفون: Aseem K Tiwari, Devi Prasad Acharya, Ravi C Dara, Dinesh Arora, Geet Aggarwal, Ganesh Singh Rawat

المصدر: Global Journal of Transfusion Medicine, Vol 2, Iss 1, Pp 29-33 (2017)

مصطلحات موضوعية: Immunochromatographic assay, rapid plasma reagin test, screening test for blood donors, serological test for syphilis, Diseases of the blood and blood-forming organs, RC633-647.5

الوصف: Introduction: Serological tests for syphilis (STS) contributed greatly to the detection of Treponema pallidum infection in blood donors and especially in those who were not identified during the medical selection. Most of the blood centers use nontreponemal tests due to lesser cost, ease of performance, and their ability to pick up early primary stage infection, despite reported high false positivity. However, increasingly large number of blood centers have begun testing with the treponemal tests such as immuno-chromatographic assay (ICA) which is possibly easier and more objective than rapid plasma reagin (RPR), albeit slightly expensive. It is with this background that we undertook a head-to-head comparison of nontreponemal test RPR with treponemal test ICA in over 10,000 consecutive blood donor samples with confirmation of all reactive and discordant samples by fluorescent treponemal antibody absorption assay (FTA-ABS) as the gold standard. Materials and Methods: The study was conducted in the department of transfusion medicine in a large tertiary care hospital in India. Consecutive blood donors from July 2014 to January 2015 were evaluated simultaneously for antitreponemal antibodies by solid phase ICA (SD BIOLINE Syphilis 3.0, Alere Medical Pvt. Ltd., USA), RPR (Immutrep RPR, Omega Diagnostics, Scotland, UK) and FTA-ABS (Biocientifica SA, FTA-ABS, Argentina). Performances of both the assays were evaluated in statistical terms. Results: A total of 10,200 donor samples were evaluated. There were 10,124 confirmed concordant negatives. Thirty-eight samples were concordant positive. Thirty-eight samples (thirty samples were ICA positive and RPR negative and eight samples were RPR positive and ICA negative) were discordant. The sensitivity of ICA was higher (93%) as compared to that of RPR (66%), whereas the specificity of RPR was higher (83%) than that of ICA (66%). Positive predictive value (PPV) of both the tests was similar while negative predictive value (NPV) of ICA was higher (86%) than that of RPR (60%). Youden's index was 0.81 for ICA whereas it was 0.62 for RPR. Conclusion: ICA has a better sensitivity than the RPR and thus seems to be more appropriate test for screening in blood donors.

وصف الملف: electronic resource

العلاقة: http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2017;volume=2;issue=1;spage=29;epage=33;aulast=TiwariTest; https://doaj.org/toc/2468-8398Test; https://doaj.org/toc/2455-8893Test

الوصول الحر: https://doaj.org/article/41e7ee81f8244effb3cb9368f70ad9e6Test

المؤلفون: Aseem K Tiwari, Prashant K Pandey, Ravi C Dara, Ganesh S Rawat, Vimarsh Raina, Richa Bhargava

المصدر: Asian Journal of Transfusion Science, Vol 9, Iss 1, Pp 65-69 (2015)

مصطلحات موضوعية: Non-treponemal test, serological test for syphilis, treponemal test, Diseases of the blood and blood-forming organs, RC633-647.5

الوصف: Context: Syphilis is a transfusion transmissible infections and it is mandatory to do serological test for syphilis (STS) on all donor blood samples. STS is usually based on detection of antibodies against the cardiolipin-lecithin antigen or against the Treponema-specific antigen. STS with good sensitivity and specificity helps enhance blood safety and consolidation of STS along with other transfusion transmittable infections such as human immunodeficiency virus, hepatitis-C virus, and hepatitis-B virus helps in reducing the errors and enhances efficiency. Aims: This study was designed to evaluate the performance of newly introduced VITROS ® syphilis Treponema pallidum agglutination (TPA) assay based on enhanced chemiluminescence principle for its analytical performance for use as a STS on donor blood samples at a tertiary care health center in National Capital Region, India. Materials and Methods: A total of 108 random blood units collected from the donors (both voluntary and replacement donors) and 28 known syphilis sero-reactive samples stored at −20°C, were used to evaluate the performance of VITROS ® syphilis TPA assay based on enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system along with its analytical performance in terms of its sensitivity, precision, cross-reactivity and interference studies. Results: VITROS ® syphilis TPA showed 100% sensitivity and specificity with precision (20 days study) of

وصف الملف: electronic resource

العلاقة: http://www.ajts.org/article.asp?issn=0973-6247;year=2015;volume=9;issue=1;spage=65;epage=69;aulast=TiwariTest; https://doaj.org/toc/0973-6247Test; https://doaj.org/toc/1998-3565Test

الوصول الحر: https://doaj.org/article/3ae8a3df361c4791bdea0f70808971efTest

المؤلفون: Ravi C Dara, Aseem K Tiwari, Devi Prasad Acharya, Dinesh Arora, Geet Aggarwal, Gunjan Bhardwaj, Gautam Kumar Gupta

المصدر: Global Journal of Transfusion Medicine, Vol 4, Iss 2, Pp 191-197 (2019)

مصطلحات موضوعية: medicine.medical_specialty, Blood transfusion, medicine.medical_treatment, syphilis, rapid plasma reagin, urologic and male genital diseases, Rapid plasma reagin, Serology, Internal medicine, medicine, fluorescent treponemal antibody-absorption assay, Treponema, Hemagglutination assay, medicine.diagnostic_test, biology, business.industry, lcsh:RC633-647.5, Transfusion medicine, Gold standard (test), lcsh:Diseases of the blood and blood-forming organs, medicine.disease, biology.organism_classification, female genital diseases and pregnancy complications, serological test for syphilis, Syphilis, automated treponema pallidum hemagglutination assay, business, immunochromatographic assay

الوصف: Introduction: Serological tests for syphilis contributed greatly to the detection of Treponema pallidum infection in blood donors and especially in those who were missed during the medical selection. Although several different treponemal assays have been evaluated in India, the debate that which treponemal test-formats would be suitable for a particular blood transfusion service is far from settled. Aims and Objectives: The study was undertaken with an objective of comparing conventional immunochromatographic assay (ICA) with new automated T. pallidum hemagglutination assay (a-TPHA) in over 10,000 consecutive blood donor samples with fluorescent treponemal antibody-absorption assay (FTA-Abs) as the gold standard to resolve the discordant samples. Materials and Methods: The study was conducted in the Department of Transfusion Medicine in a large tertiary care hospital in India. Consecutive blood donors from July 2014 to January 2015 were evaluated simultaneously for antitreponemal antibodies by solid-phase ICA (SD BIOLINE Syphilis 3.0, Alere Medical Pvt. Ltd., USA), a-TPHA (Immucor Diagnostics, USA) and FTA-Abs (Biocientifica SA, FTA-Abs, Argentina). Performances of both the assays were evaluated using statistical tools. Results: A total of 10,129 donor samples were evaluated. A total of 113 (1.1%) donor samples were reactive for syphilis; either on ICA or a-TPHA or both. Ninety-one of these 113 reactive samples were reactive on FTA-Abs, giving seroprevalance of syphilis as 0.9%. a-TPHA was superior to ICA in terms of sensitivity, specificity, positive predictive value, negative predictive value, and Youden's index. Conclusion: The new “a-TPHA” is a superior assay to ICA as the screening assay for syphilis in blood donors.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f62cb98b09a87dd717342009059e65a7Test
http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2019;volume=4;issue=2;spage=191;epage=197;aulastTest=

المؤلفون: Ravi C Dara, Ganesh Rawat, Aseem K Tiwari, Geet Aggarwal, Devi Prasad Acharya, Dinesh Arora

المصدر: Global Journal of Transfusion Medicine, Vol 2, Iss 1, Pp 29-33 (2017)

مصطلحات موضوعية: 030213 general clinical medicine, medicine.medical_specialty, 030204 cardiovascular system & hematology, urologic and male genital diseases, Gastroenterology, Rapid plasma reagin, Serology, rapid plasma reagin test, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Internal medicine, medicine, Rpr positive, screening test for blood donors, biology, medicine.diagnostic_test, business.industry, lcsh:RC633-647.5, Transfusion medicine, Gold standard (test), lcsh:Diseases of the blood and blood-forming organs, Fluorescent Treponemal Antibody Absorption, medicine.disease, serological test for syphilis, biology.protein, Syphilis, Antibody, business, Immunochromatographic assay

الوصف: Introduction: Serological tests for syphilis (STS) contributed greatly to the detection of Treponema pallidum infection in blood donors and especially in those who were not identified during the medical selection. Most of the blood centers use nontreponemal tests due to lesser cost, ease of performance, and their ability to pick up early primary stage infection, despite reported high false positivity. However, increasingly large number of blood centers have begun testing with the treponemal tests such as immuno-chromatographic assay (ICA) which is possibly easier and more objective than rapid plasma reagin (RPR), albeit slightly expensive. It is with this background that we undertook a head-to-head comparison of nontreponemal test RPR with treponemal test ICA in over 10,000 consecutive blood donor samples with confirmation of all reactive and discordant samples by fluorescent treponemal antibody absorption assay (FTA-ABS) as the gold standard. Materials and Methods: The study was conducted in the department of transfusion medicine in a large tertiary care hospital in India. Consecutive blood donors from July 2014 to January 2015 were evaluated simultaneously for antitreponemal antibodies by solid phase ICA (SD BIOLINE Syphilis 3.0, Alere Medical Pvt. Ltd., USA), RPR (Immutrep RPR, Omega Diagnostics, Scotland, UK) and FTA-ABS (Biocientifica SA, FTA-ABS, Argentina). Performances of both the assays were evaluated in statistical terms. Results: A total of 10,200 donor samples were evaluated. There were 10,124 confirmed concordant negatives. Thirty-eight samples were concordant positive. Thirty-eight samples (thirty samples were ICA positive and RPR negative and eight samples were RPR positive and ICA negative) were discordant. The sensitivity of ICA was higher (93%) as compared to that of RPR (66%), whereas the specificity of RPR was higher (83%) than that of ICA (66%). Positive predictive value (PPV) of both the tests was similar while negative predictive value (NPV) of ICA was higher (86%) than that of RPR (60%). Youden's index was 0.81 for ICA whereas it was 0.62 for RPR. Conclusion: ICA has a better sensitivity than the RPR and thus seems to be more appropriate test for screening in blood donors.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5169614fd9c4d9ef86e8108e6651b73bTest
http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2017;volume=2;issue=1;spage=29;epage=33;aulast=TiwariTest

المؤلفون: Richa Bhargava, Aseem K Tiwari, Ravi C Dara, Prashant Pandey, Vimarsh Raina, Ganesh Rawat

المصدر: Asian Journal of Transfusion Science, Vol 9, Iss 1, Pp 65-69 (2015)
Asian Journal of Transfusion Science

مصطلحات موضوعية: law.invention, Serology, Antigen, law, medicine, Immunology and Allergy, Chemiluminescence, Chemiluminescence assay, Immunodiagnostics, Treponema, biology, business.industry, lcsh:RC633-647.5, Hematology, lcsh:Diseases of the blood and blood-forming organs, biology.organism_classification, medicine.disease, treponemal test, Non-treponemal test, serological test for syphilis, Immunology, biology.protein, Syphilis, Original Article, Antibody, business

الوصف: Context: Syphilis is a transfusion transmissible infections and it is mandatory to do serological test for syphilis (STS) on all donor blood samples. STS is usually based on detection of antibodies against the cardiolipin-lecithin antigen or against the Treponema-specific antigen. STS with good sensitivity and specificity helps enhance blood safety and consolidation of STS along with other transfusion transmittable infections such as human immunodeficiency virus, hepatitis-C virus, and hepatitis-B virus helps in reducing the errors and enhances efficiency. Aims: This study was designed to evaluate the performance of newly introduced VITROS ® syphilis Treponema pallidum agglutination (TPA) assay based on enhanced chemiluminescence principle for its analytical performance for use as a STS on donor blood samples at a tertiary care health center in National Capital Region, India. Materials and Methods: A total of 108 random blood units collected from the donors (both voluntary and replacement donors) and 28 known syphilis sero-reactive samples stored at −20°C, were used to evaluate the performance of VITROS ® syphilis TPA assay based on enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system along with its analytical performance in terms of its sensitivity, precision, cross-reactivity and interference studies. Results: VITROS ® syphilis TPA showed 100% sensitivity and specificity with precision (20 days study) of

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c17d00588322f4ab241cd1d0b1b8bff9Test
http://www.ajts.org/article.asp?issn=0973-6247;year=2015;volume=9;issue=1;spage=65;epage=69;aulast=TiwariTest