دورية أكاديمية
Characteristic response of striatal astrocytes to dopamine depletion
| العنوان: | Characteristic response of striatal astrocytes to dopamine depletion |
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| المؤلفون: | Yao-Feng Zhu, Wei-Ping Wang, Xue-Feng Zheng, Zhi Chen, Tao Chen, Zi-Yun Huang, Lin-Ju Jia, Wan-Long Lei |
| المصدر: | Neural Regeneration Research, Vol 15, Iss 4, Pp 724-730 (2020) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2020 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | 6-hydroxydopamine, astrocyte, dopamine depletion, dopaminergic neurons, parkinson’s disease, s100b, stat3, striatum, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | Astrocytes and astrocyte-related proteins play important roles in maintaining normal brain function, and also regulate pathological processes in brain diseases and injury. However, the role of astrocytes in the dopamine-depleted striatum remains unclear. A rat model of Parkinson’s disease was therefore established by injecting 10 μL 6-hydroxydopamine (2.5 μg/μL) into the right medial forebrain bundle. Immunohistochemical staining was used to detect the immunoreactivity of glial fibrillary acidic protein (GFAP), calcium-binding protein B (S100B), and signal transducer and activator of transcription 3 (STAT3) in the striatum, and to investigate the co-expression of GFAP with S100B and STAT3. Western blot assay was used to measure the protein expression of GFAP, S100B, and STAT3 in the striatum. Results demonstrated that striatal GFAP-immunoreactive cells had an astrocytic appearance under normal conditions, but that dopamine depletion induced a reactive phenotype with obvious morphological changes. The normal striatum also contained S100B and STAT3 expression. S100B-immunoreactive cells were uniform in the striatum, with round bodies and sparse, thin processes. STAT3-immunoreactive cells presented round cell bodies with sparse processes, or were darkly stained with a large cell body. Dopamine deprivation induced by 6-hydroxydopamine significantly enhanced the immunohistochemical positive reaction of S100B and STAT3. Normal striatal astrocytes expressed both S100B and STAT3. Striatal dopamine deprivation increased the number of GFAP/S100B and GFAP/STAT3 double-labeled cells, and increased the protein levels of GFAP, S100B, and STAT3. The present results suggest that morphological changes in astrocytes and changes in expression levels of astrocyte-related proteins are involved in the pathological process of striatal dopamine depletion. The study was approved by Animal Care and Use Committee of Sun Yat-sen University, China (Zhongshan Medical Ethics 2014 No. 23) on September 22, 2014. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1673-5374 |
| العلاقة: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=4;spage=724;epage=730;aulast=ZhuTest; https://doaj.org/toc/1673-5374Test; https://doaj.org/article/216a67946c72464ea09f838495102f16Test |
| DOI: | 10.4103/1673-5374.266917 |
| الإتاحة: | https://doi.org/10.4103/1673-5374.266917Test https://doaj.org/article/216a67946c72464ea09f838495102f16Test |
| رقم الانضمام: | edsbas.B524B4CB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 16735374 |
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| DOI: | 10.4103/1673-5374.266917 |