دورية أكاديمية

Prevalence and virulence factors of Candida spp. associated with blow flies

التفاصيل البيبلوغرافية
العنوان: Prevalence and virulence factors of Candida spp. associated with blow flies
المؤلفون: Wimonrat GunTang, Nathamon Kamonvoradej, Chitchanok Chomchat, Sangrawee Suriyakan, Sangob Sanit, Jintana Wongwigkarn, Nophawan Bunchu, Damrongpan Thongwat, Supaporn Lamlertthon
المصدر: Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 5, Pp 428-431 (2017)
بيانات النشر: Wolters Kluwer Medknow Publications, 2017.
سنة النشر: 2017
المجموعة: LCC:Arctic medicine. Tropical medicine
LCC:Biology (General)
مصطلحات موضوعية: Blow flies, Candida albicans, β-Hemolysin, Phospholipase, Antifungal resistance, Arctic medicine. Tropical medicine, RC955-962, Biology (General), QH301-705.5
الوصف: Objective: To investigate the prevalence of Candida spp. and the virulence factors of Candida albicans (C. albicans) isolated from external surfaces of blow flies collected from Mae Sot, Tak Province, Thailand. Methods: The blow flies were collected by sterile sweep nets from three areas in Mae Sot. Yeast isolation was first performed on Sabouraud dextrose agar (SDA) supplemented with chloramphenicol and cycloheximide. The yeast isolates were then identified by using chromogenic agar, a yeast identification test kit, a germ tube formation test and a carbohydrate utilization test. The β-hemolysis was determined on 7% sheep blood agar, while phospholipase activity was measured on SDA agar supplemented with 10% egg yolk suspension. Antifungal susceptibility testing was determined by broth micro-dilution testing against ketoconazole and amphotericin B. Results: The prevalence rate of Candida spp. on the external surfaces of the blow flies was 78.1%. All C. albicans isolated from the blow fly demonstrated β-hemolysin and potent phospholipase activities and 47.1% of C. albicans were resistant to ketoconazole with MIC values 128 μg/mL. Conclusions: The results indicate that blow flies could play an essential role in the transmission of potentially pathogenic and antifungal resistant C. albicans into the environment. Further investigation on other virulence factors and genetic relatedness among isolates from the blow flies, the environment and clinical specimens is required to confirm this role.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2221-1691
العلاقة: http://www.sciencedirect.com/science/article/pii/S2221169116304324Test; https://doaj.org/toc/2221-1691Test
DOI: 10.1016/j.apjtb.2017.01.014
الوصول الحر: https://doaj.org/article/9db36eefdf8946e68c514474479e44d4Test
رقم الانضمام: edsdoj.9db36eefdf8946e68c514474479e44d4
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22211691
DOI:10.1016/j.apjtb.2017.01.014