دورية أكاديمية
Rapid NMR Assignments of Proteins by Using Optimized Combinatorial Selective Unlabeling
| العنوان: | Rapid NMR Assignments of Proteins by Using Optimized Combinatorial Selective Unlabeling |
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| المؤلفون: | Dubey, Abhinav, Kadumuri, Rajashekar Varma, Jaipuria, Garima, Vadrevu, Ramakrishna, Atreya, Hanudatta S |
| بيانات النشر: | WILEY-V C H VERLAG GMBH |
| سنة النشر: | 2016 |
| المجموعة: | Indian Institute of Science, Bangalore: ePrints@IIsc |
| مصطلحات موضوعية: | NMR Research Centre (Formerly SIF), Others |
| الوصف: | A new approach for rapid resonance assignments in proteins based on amino acid selective unlabeling is presented. The method involves choosing a set of multiple amino acid types for selective unlabeling and identifying specific tripeptides surrounding the labeled residues from specific 2D NMR spectra in a combinatorial manner. The methodology directly yields sequence specific assignments, without requiring a contiguously stretch of amino acid residues to be linked, and is applicable to deuterated proteins. We show that a 2D N-15,H-1]HSQC spectrum with two 2D spectra can result in approximate to 50% assignments. The methodology was applied to two proteins: an intrinsically disordered protein (12kDa) and the 29kDa (268 residue) -subunit of Escherichia coli tryptophan synthase, which presents a challenging case with spectral overlaps and missing peaks. The method can augment existing approaches and will be useful for applications such as identifying active-site residues involved in ligand binding, phosphorylation, or protein-protein interactions, even prior to complete resonance assignments. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://eprints.iisc.ernet.in/53551/1/Che_Pub_Soc_17-4_334_2016.pdfTest; Dubey, Abhinav and Kadumuri, Rajashekar Varma and Jaipuria, Garima and Vadrevu, Ramakrishna and Atreya, Hanudatta S (2016) Rapid NMR Assignments of Proteins by Using Optimized Combinatorial Selective Unlabeling. In: CHEMBIOCHEM, 17 (4). pp. 334-340. |
| DOI: | 10.1002/cbic.201500513 |
| الإتاحة: | https://doi.org/10.1002/cbic.201500513Test http://eprints.iisc.ernet.in/53551Test/ http://eprints.iisc.ernet.in/53551/1/Che_Pub_Soc_17-4_334_2016.pdfTest |
| رقم الانضمام: | edsbas.159B149E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/cbic.201500513 |
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