Determination of the antiretroviral drug tenofovir in plasma from HIV-infected adults by ultrafast isotope dilution MALDI-triple quadrupole tandem mass spectrometry
| العنوان: | Determination of the antiretroviral drug tenofovir in plasma from HIV-infected adults by ultrafast isotope dilution MALDI-triple quadrupole tandem mass spectrometry |
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| المؤلفون: | Rachel D. Scheuer, Marchina E. van der Ende, Theo M. Luider, Rob A. Gruters, Jeroen J. A. van Kampen, Roland J. W. Meesters |
| المساهمون: | Neurology, Virology, Internal Medicine |
| المصدر: | Journal of Mass Spectrometry, 46(3), 282-289. Wiley-Blackwell |
| بيانات النشر: | Wiley-Blackwell, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Adult, Isotope dilution method, Anti-HIV Agents, Organophosphonates, HIV Infections, Isotope dilution, Tandem mass spectrometry, Sensitivity and Specificity, SDG 3 - Good Health and Well-being, Tandem Mass Spectrometry, Humans, Tenofovir, Triglycerides, Spectroscopy, Detection limit, Chromatography, Chemistry, Adenine, Selected reaction monitoring, Reproducibility of Results, Triple quadrupole mass spectrometer, Matrix-assisted laser desorption/ionization, Isotope Labeling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Linear Models, Quantitative analysis (chemistry) |
| الوصف: | A new and reliable mass spectrometric method using an isotope dilution method in combination with matrix-assisted laser desorption/ionization-triple quadrupole tandem mass spectrometry (ID-MALDI-QqQ-MS/MS) has been developed and validated for the determination of concentrations of the antiretroviral drug tenofovir (TNV) in plasma from HIV-infected adults. The advantage of this new method is that (1) the method is ultrafast and(2) can be applied for high-throughput measurement of TNV in plasma. The method is based on a simple plasma deproteinization step in combination with the use of [adenine-(13)C(5)]-TNV as the internal standard. TNV and [adenine-(13)C(5)]-TNV were monitored by multiple reaction monitoring using the transition m/z 288.0 -> 176.2 and m/z 293.2 -> 181.2 for TNV and [adenine-(13)C(5)]-TNV, respectively. The method was validated according to the most recent FDA guidelines for the development and validation of (new) bio-analytical assays. Validated method parameters were: linearity, accuracy, precision and stability of the method. The lowest limit of quantification was 0.10 mu mol/l, whereas the limit of detection determined at a signal-to-noise ratio (S/N = 3 : 1) in pooled drug free human control plasma was 0.04 mu mol/l. The validated method was successfully applied and tested for its clinical feasibility by the analysis of plasma samples from selected HIV-infected adults receiving the prodrug tenofovir disoproxil fumarate. Observed plasma TNV concentrations ranged between 0.11 and 0.76 mu mol/l and measured plasma TNV concentrations were within the therapeutically relevant concentration range. Copyright (C) 2011 John Wiley & Sons, Ltd. |
| تدمد: | 1096-9888 1076-5174 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::668b3d745c9eda3fa39c49a2b7040991Test https://pure.eur.nl/en/publications/cbd8d231-3dff-4d1e-8ce5-d509a7e03934Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....668b3d745c9eda3fa39c49a2b7040991 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969888 10765174 |
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