Interaction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation
| العنوان: | Interaction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation |
|---|---|
| المؤلفون: | Frank Steiniger, Walter Richter, Lauren Mashburn-Warren, Marvin Whiteley, Jörg Howe, Klaus Brandenburg, Manfred Roessle, Patrick Garidel |
| المصدر: | Molecular microbiology 69, 491-502 (2008). doi:10.1111/j.1365-2958.2008.06302.x Molecular Microbiology |
| بيانات النشر: | Wiley-Blackwell, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Lipopolysaccharides, Cell signaling, Lipopolysaccharide, Membrane lipids, Quinolones, Biology, medicine.disease_cause, Microbiology, Cell membrane, Membrane Lipids, chemistry.chemical_compound, ddc:570, medicine, Molecular Biology, Research Articles, Phospholipids, Molecular Structure, Pseudomonas aeruginosa, Cell Membrane, Cryoelectron Microscopy, biology.organism_classification, Cell biology, Quorum sensing, medicine.anatomical_structure, chemistry, Liposomes, Bacterial outer membrane, Bacteria |
| الوصف: | Bacteria have evolved elaborate communication strategies to co-ordinate their group activities, a process termed quorum sensing (QS). Pseudomonas aeruginosa is an opportunistic pathogen that utilizes QS for diverse activities, including disease pathogenesis. P. aeruginosa has evolved a novel communication system in which the signal molecule 2-heptyl-3-hydroxy-4-quinolone (Pseudomonas Quinolone Signal, PQS) is trafficked between cells via membrane vesicles (MVs). Not only is PQS packaged into MVs, it is required for MV formation. Although MVs are involved in important biological processes aside from signalling, the molecular mechanism of MV formation is unknown. To provide insight into the molecular mechanism of MV formation, we examined the interaction of PQS with bacterial lipids. Here, we show that PQS interacts strongly with the acyl chains and 4'-phosphate of bacterial lipopolysaccharide (LPS). Using PQS derivatives, we demonstrate that the alkyl side-chain and third position hydroxyl of PQS are critical for these interactions. Finally, we show that PQS stimulated purified LPS to form liposome-like structures. These studies provide molecular insight into P. aeruginosa MV formation and demonstrate that quorum signals serve important non-signalling functions. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::537141fd8c447922c3f953c78e1fbc62Test https://bib-pubdb1.desy.de/record/87034Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....537141fd8c447922c3f953c78e1fbc62 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |