التفاصيل البيبلوغرافية
| العنوان: |
Regulation of type I plasminogen activator inhibitor in human gingival fibroblasts with cyclosporine A. |
| المؤلفون: |
Ho, Y‐C1 (AUTHOR), Lin, H‐J2 (AUTHOR), Tsai, C‐H3 (AUTHOR), Chang, Y‐C2,4 (AUTHOR) |
| المصدر: |
Oral Diseases. May2010, Vol. 16 Issue 4, p396-401. 6p. 4 Graphs. |
| مصطلحات موضوعية: |
*CYCLOSPORINE, *PLASMINOGEN, *FIBRINOLYTIC agents, *GINGIVAL fluid, *PROTEIN kinases, *CYTOKINES, *MESSENGER RNA, *POLYMERASE chain reaction |
| مستخلص: |
Oral Diseases (2010) 16, 396–401 Objectives: Cyclosporine A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of gingival overgrowth. Type I plasminogen activator inhibitor (PAI-1) has shown to play an important role in CsA-induced gingival overgrowth. However, little is known about whether factors can modulate CsA-induced PAI-1 expression. Methods: Cytotoxicity, reverse transcriptase-polymerase chain reaction, and enzyme-linked immunosorbent assay were used to investigate the effects of Human gingival fibroblasts (HGFs) exposed to CsA. In addition, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, interlukin-1α, tumor necrosis factor-α, mitogen-activated protein kinase kinase (MEK) inhibitor U0126, signal-regulated protein kinase (ERK) inhibitor PD98059 and cell-permeable glutathione precursor N-acetyl- L-cysteine (NAC) were added to test how they modulated the effects of CsA-induced PAI-1 expression. Results: The concentration of CsA higher than 500 ng ml−1 demonstrated cytotoxicity to HGFs ( P < 0.05). Periodontal pathogens as well as proinflammatory cytokines were found to increase the CsA-induced PAI-1 mRNA and protein expression ( P < 0.05). Pharmacological agents NAC, U0126, and PD98059 were found to decrease the CsA-induced PAI-1 mRNA and protein expression ( P < 0.05). Conclusions: Cyclosporine A (CsA) may predispose to gingival overgrowth under inflammatory environments. The regulation of PAI-1 expression induced by CsA might be critically related with the intracellular glutathione and the ERK-MAPK pathway. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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