التفاصيل البيبلوغرافية
| العنوان: |
Cytotoxic herpes simplex type 2-specific, DQ0602-restricted CD4+ T-cell clones show alloreactivity to DQ0601. |
| المؤلفون: |
Reichstetter, Sandra bkwok@benaroyaresearch.org, Standifer, Nathan E., Geubtner, Kelly A., Liu, Andrew W., Agar, Stacy L., Kwok, William W.1 sreichstetter@earthlink.net |
| المصدر: |
Immunology. Mar2006, Vol. 117 Issue 3, p350-357. 8p. 2 Charts, 8 Graphs. |
| مصطلحات موضوعية: |
*HERPES simplex, *MAJOR histocompatibility complex, *TRANSPLANTATION of organs, tissues, etc., *IMMUNOREGULATION, *NUCLEOTIDE sequence, *T-cell receptor genes, *CYTOKINES, *T cells |
| مستخلص: |
Alloreactivity is one of the most serious problems in organ transplantation. It has been hypothesized that pre-existing alloreactive T cells are actually cross-reacting cells that have been primed by the autologous major histocompatibility complex (MHC) and a specific peptide. CD8+ cytotoxic T lymphocytes that are alloreactive and recognize a virus-peptide that is presented by the autologous MHC have been reported. Here we demonstrate a cross-reactivity that exists between DQ0602 restricted, herpes simplex type 2 VP16 40–50 specific CD4+ T-cell clones, which can be alloreactive to DQ0601. Though most of the DQ0602 restricted T-cell clones we isolated from two different donors were not alloreactive, weakly cross-reacting T-cell clones could be isolated from both donors. Two strongly cross-reacting T-cell clones with high affinity interaction of their T-cell receptor (TCR) with both DQ0602/VP16 40–50 and DQ0601 could be isolated from one donor. DNA sequencing of the a fragment of the Vβ gene used in their TCR confirmed that these two T cells indeed are two independent clones. These clones are cytotoxic and produce cytokines of a T helper 2-like pattern. Possible implications in a DR-matched transplantation setting are discussed. [ABSTRACT FROM AUTHOR] |
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