دورية أكاديمية
Evaluation of a method allowing preservation of fresh lymph nodes for flow cytometric immunophenotyping
| العنوان: | Evaluation of a method allowing preservation of fresh lymph nodes for flow cytometric immunophenotyping |
|---|---|
| المؤلفون: | Brincat, Patricia, DeGaetano, James, Donaldson, Craig |
| بيانات النشر: | Wiley Online Library |
| سنة النشر: | 2012 |
| المجموعة: | University of Malta: OAR@UM / L-Università ta' Malta |
| مصطلحات موضوعية: | Lymph nodes, Lymphomas -- Diagnosis, Flow cytometry, Cryopreservation of organs, tissues, etc |
| الوصف: | Background: Flow cytometric immunophenotyping (FCI) of lymph nodes (LN) requires fresh unfixed tissue, with analysis being carried out within few hours post surgery. This study evaluated a novel method for fresh LN preservation, in order to allow histomorphology-based FCI. Methods: This study was carried out prospectively on 30 LN with suspected involvement by haematolymphoid neoplasms (HLN). FCI was performed on each fresh and post cryopreserved LN cell suspension. Percentage positivities (PP) and mean fluorescent intensities (MFI) were calculated on both preparations for a combination of T and B-lymphoid antigens together with viability. Results: The cryopreservation method applied in this study did not affect significantly PP and had minor impact on MFI of the tested antigens. Overall, there was minimal decrease in PP and MFI on the cryopreserved cells when compared with fresh cells, for most antigens with only a mild increase in apoptotic cells. However, these changes were not diagnostically significant, since both reactive processes and HLN present within analyzed LN could be identified and differentiated. Viability was more than 75% for all cryopreserved LN composed of haematolymphoid cells. Conclusion: The method presented in this study confers the possibility of storing fresh LN biopsies for later FCI, thus allowing a morphology-based immunophenotypic approach. This would allow a more sensitive, specific, and cost-effective management of LN specimens, whilst maintaining the important benefits provided by FCI. ; peer-reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Brincat, P., Degaetano, J., & Donaldson, C. (2012). Evaluation of a method allowing preservation of fresh lymph nodes for flow cytometric immunophenotyping. Cytometry Part B: Clinical Cytometry, 82(4), 245-251.; https://www.um.edu.mt/library/oar/handle/123456789/45863Test |
| DOI: | 10.1002/cyto.b.21021 |
| الإتاحة: | https://doi.org/10.1002/cyto.b.21021Test https://www.um.edu.mt/library/oar/handle/123456789/45863Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. |
| رقم الانضمام: | edsbas.91ED3A33 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/cyto.b.21021 |
|---|