دورية أكاديمية
Interaction of [(VO)-O-IV(acac)(2)] with Human Serum Transferrin and Albumin
| العنوان: | Interaction of [(VO)-O-IV(acac)(2)] with Human Serum Transferrin and Albumin |
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| المؤلفون: | Correia, Isabel, Chorna, Ielyzaveta, Cavaco, Isabel, Roy, Somnath, Kuznetsov, Maxim L., Ribeiro, Nadia, Justino, Goncalo, Marques, Fernanda, Santos-Silva, Teresa, Santos, Marino F. A., Hugo, M. Santos E., Capelo, Jose L., Doutch, James, Pessoa, Joao Costa |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Universidade do Algarve: Sapienta |
| مصطلحات موضوعية: | Heteroleptic oxidovanadium(Iv) compounds, Antidiabetic vanadium complexes, Nuclear double-resonance, Ray solution scattering, Red-blood-cells, Crystal-structure, Oxovanadium(Iv) complexes, N-lobe, Tyrosine phosphorylation, Coordination chemistry |
| الوصف: | VO(acac)(2)] is a remarkable vanadium compound and has potential as a therapeutic drug. It is important to clarify how it is transported in blood, but the reports addressing its binding to serum proteins have been contradictory. We use several spectroscopic and mass spectrometric techniques (ESI and MALDI-TOF), small-angle X-ray scattering and size exclusion chromatography (SEC) to characterize solutions containing [VO(acac)(2)] and either human serum apotransferrin (apoHTF) or albumin (HSA). DFT and modeling protein calculations are carried out to disclose the type of binding to apoHTF. The measured circular dichroism spectra, SEC and MALDI-TOF data clearly prove that at least two VOacac moieties may bind to apoHTF, most probably forming [(VO)-O-IV(acac)(apoHTF)] complexes with residues of the HTF binding sites. No indication of binding of [VO(acac)(2)] to HSA is obtained. We conclude that (VO)-O-IV-acac species may be transported in blood by transferrin. At very low complex concentrations speciation calculations suggest that [(VO)(apoHTF)] species form. ; Fundacao para a Ciencia e Tecnologia (FCT), Portugal [ RECI/QEQMED/0330/2012, PTDC/QEQ-MED/1902/2014] ; FCT [IF/00100/2013, IF/00007/2015] ; PROTEOMASS Scientific Society ; UCIBIO, Unidade de Ciencias Biomoleculares Aplicadas [UID/Multi/04378/2013] ; ERDF [POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265] ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1861-4728 1861-471X |
| العلاقة: | info:eu-repo/grantAgreement/FCT/5876/147266/PT; info:eu-repo/grantAgreement/FCT/5876/147216/PT; info:eu-repo/grantAgreement/FCT/3599-PPCDT/126438/PT; info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F77894%2F2011/PT; http://hdl.handle.net/10400.1/13033Test |
| DOI: | 10.1002/asia.201700469 |
| الإتاحة: | https://doi.org/10.1002/asia.201700469Test http://hdl.handle.net/10400.1/13033Test |
| حقوق: | openAccess ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.2DA9B630 |
| قاعدة البيانات: | BASE |
| تدمد: | 18614728 1861471X |
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| DOI: | 10.1002/asia.201700469 |