An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistance against grapevine downy mildew
| العنوان: | An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistance against grapevine downy mildew |
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| المؤلفون: | Frédéric Domergue, Vincent Ferrières, Marielle Adrian, Christian Gauvrit, Sophie Trouvelot, Jérôme Joubès, Franck Paris, Laurent Legentil, Frank Jamois, Xavier Daire, Yuko Krzyzaniak |
| المساهمون: | Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Région Bretagne, Conseil Régional de Bourgogne, Institut des Sciences Chimiques de Rennes ( ISCR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Ecole Nationale Supérieure de Chimie de Rennes-Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Physiologia Plantarum Physiologia Plantarum, Wiley, 2016, 156 (3), pp.338-350. ⟨10.1111/ppl.12394⟩ Physiologia Plantarum, 2016, 156 (3), pp.338-350. 〈10.1111/ppl.12394〉 Physiologia Plantarum, 2016, 156 (3), pp.338-350. ⟨10.1111/ppl.12394⟩ |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Ethylene Oxide, 0106 biological sciences, 0301 basic medicine, Sucrose, Physiology, Plant Science, Biology, Disaccharides, [ CHIM ] Chemical Sciences, 01 natural sciences, Fluorescence, Diffusion, Cell wall, Surface-Active Agents, 03 medical and health sciences, Laminarin, chemistry.chemical_compound, Pulmonary surfactant, Polysaccharides, Botany, Genetics, [CHIM]Chemical Sciences, Vitis, Carbon Radioisotopes, Disease Resistance, Plant Diseases, Plant Stomata, food and beverages, Cell Biology, General Medicine, Penetration (firestop), Trichome, Kinetics, Horticulture, Cholesterol, 030104 developmental biology, Oomycetes, Plant cuticle, chemistry, Waxes, Downy mildew, 010606 plant biology & botany |
| الوصف: | International audience; Some β-1,3-glucans and particularly sulfated laminarin (PS3) are known as resistance inducers (RIs) in grapevine against the downy mildew. However, their efficacy in vineyard is still often too low, which might be caused by a limited penetration through the leaf cuticle following spray application. We used (14) C-sucrose uptake experiments with grapevine leaves in order to select a surfactant as saccharide penetration enhancer. Our results showed that though sucrose foliar uptake was low, it was strongly enhanced by Dehscofix CO125 (DE), a highly ethoxylated surfactant. Fluorescent saccharides were then produced and laser scanning microscopy was used to analyze their foliar diffusion pattern in Arabidopsis thaliana and grapevine. Interestingly, sucrose and PS3 were seemingly able to penetrate the leaf cuticle only when formulated with DE. Diffusion could preferentially occur via stomata, anticlinal cell walls and trichomes. In grapevine, PS3 penetration rate was much higher on the stomateous abaxial surface of the leaf than on the adaxial surface. Finally, using DE allowed a higher level of downy mildew control by PS3, which corroborated diffusion observations. Our results have practical consequences for the improvement of treatments with saccharidic inducers on grape. That is, formulation of such RIs plays a critical role for their cuticular diffusion and consequently their efficacy. Also, spray application should preferentially target the abaxial surface of the leaves in order to maximize their penetration |
| تدمد: | 0031-9317 1399-3054 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b30b81765fce3726899832833f21645Test https://doi.org/10.1111/ppl.12394Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9b30b81765fce3726899832833f21645 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00319317 13993054 |
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