التفاصيل البيبلوغرافية
| العنوان: |
New polymorphic HLA‐DR epitopes recognized by three monoclonal antibodies produced against DR103 transfected L cells |
| المؤلفون: |
Cayrol, Corinne, Moro, Franĉoise, Sommer, Evelyne, Tkaczuk, Jean, Ohayon, Elie, Cambon‐Thomsen, Anne |
| المصدر: |
Tissue Antigens ; volume 40, issue 4, page 197-203 ; ISSN 0001-2815 1399-0039 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
1992 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Production of monoclonal antibodies directed against polymorphic epitopes of HLA class II molecules using whole human cells as immunogen has often proved ineffective, because most of the antibodies produced are directed against non‐MHC human cell surface molecules. One approach to overcome this problem is the use of transfected mouse L cells expressing a single HLA class II allele as immunogen. By immunizing C3H mice with DR103‐transfected L cells, we obtained 3 mAb, OHA TM901, OHA TM902, and OHA TM903, that recognize different polymorphic epitopes of the HLA‐DR molecule. The molecular specificities of the 3 mAb were determined on a large panel of B‐lymphoblastoid cell lines (B‐LCL), peripheral blood cells and HLA class II transfectants from the XIth International Histocompatibility Workshop. Interestingly, the 3 polymorphic mAb detect new HLA‐DR epitopes shared by several specificities: OHA TM901 reacts with DR1 (DR101, DR103), DR9 (DR901) and DR10 (DR1001) molecules; OHA TM902 recognizes the same molecules but also DR8 (DR801, 802, 803); OHA TM903 reacts with all DR types except DR3 (DR301, 302), DR7 (DR701, 702) and DR52. Surprisingly, OHA TM901 reacts with DR9 transfectants and B‐LCL but not with DR9 peripheral blood lymphocytes. Biochemical analyses indicate that the 3 mAb immunoprecipitate HLA‐DR products and react in western blots with DR α/β‐dimer but not with free α‐ or β‐chains. This study shows that transfected L cells are very useful tools for the production and the fine characterization of mAb recognizing polymorphic epitopes of HLA class II molecules. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/j.1399-0039.1992.tb02045.x |
| الإتاحة: |
https://doi.org/10.1111/j.1399-0039.1992.tb02045.xTest |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.F9904AA5 |
| قاعدة البيانات: |
BASE |
