Seroepidemiology of human group C rotavirus in the UK
| العنوان: | Seroepidemiology of human group C rotavirus in the UK |
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| المؤلفون: | S.J. Cooke, V. L. A. James, E. O. Caul, Paul R. Lambden, Ian N. Clarke |
| المصدر: | Journal of Medical Virology. 52:86-91 |
| بيانات النشر: | Wiley, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | biology, viruses, virus diseases, Reoviridae, biology.organism_classification, medicine.disease_cause, Group A, Virology, Virus, law.invention, Serology, Infectious Diseases, Antigen, law, Rotavirus, Recombinant DNA, medicine, biology.protein, Antibody |
| الوصف: | The gene coding for the major inner capsid protein VP6 of human group C rotavirus was cloned into baculovirus using the pBlueBac2 vector and expressed in insect cells. When cultured in High Five cells, VP6 was expressed at a high level and exported to the cell culture medium. Purified VP6 was used to immunise rabbits. Hyperimmune rabbit serum, which reacted with native human group C rotavirus in infected cells, was used to develop and optimise an EIA for the detection of antibodies to group C rotavirus using the recombinant VP6 as a source of antigen. In a local epidemiological survey of 1000 sera grouped by age, an average of 43% of samples were found to have antibodies to human group C rotavirus with the highest proportion (66%) in the 71-75 year age group. In comparison, 97% of adults and 85% of children had antibodies to recombinant VP6 from the bovine RF strain of group A rotavirus. These results suggest that infection with human group C rotavirus is a common occurrence despite the apparent rarity of reports of human group C rotavirus in clinical samples from patients with gastroenteritis. |
| تدمد: | 1096-9071 0146-6615 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::8cd93dd6c7d60dc3efbee113be25ede0Test https://doi.org/10.1002Test/(sici)1096-9071(199705)52:1<86::aid-jmv14>3.0.co;2-z |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........8cd93dd6c7d60dc3efbee113be25ede0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10969071 01466615 |
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