التفاصيل البيبلوغرافية
| العنوان: |
Dendritic Cells Pulsed With Apoptotic Squamous Cell Carcinoma Have Anti‐Tumor Effects When Combined With Interleukin‐2 |
| المؤلفون: |
Son, Young‐Ik, Mailliard, Robbie B., Watkins, Simon C., Lotze, Michael T. |
| المصدر: |
The Laryngoscope ; volume 111, issue 8, page 1472-1478 ; ISSN 0023-852X 1531-4995 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2001 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Objectives Dendritic cells, the most potent of the antigen‐presenting cells, have been widely studied as a promising tool for antitumor immunotherapies. However, little has been determined about the efficacy of dendritic cell–based therapy for the treatment of squamous cell carcinoma (SCC) because there are no known SCC‐specific antigens. Recent reports indicate that dendritic cells can acquire antigens in the form of apoptotic cells and induce cytotoxic T‐lymphocyte responses. The aim of this study was to test the feasibility of adoptive dendritic cell immunotherapy against SCC by using apoptotic tumor cells as a source of tumor antigens. Study Design A poorly immunogenic SCC line KLN 205 was used to make subcutaneous tumors on the flank of DBA2/J syngeneic mice. Bone marrow–derived dendritic cells were pulsed with ultraviolet B–irradiated (apoptotic) KLN 205 cells in vitro and transferred to the opposite flank subcutaneously. Some of the animals received simultaneous intraperitoneal injections of low‐dose interleukin‐2. Results When combined with interleukin‐2, adoptive transfers of dendritic cells that were pulsed with apoptotic SCC significantly suppressed the tumor growth ( P <.001) without notable side effects. Splenic T cells of treated mice produced greater amounts of interferon‐γ when restimulated with the relevant tumor ( P <.001) as compared with control groups, indicative of an effective T‐cell–mediated systemic immune response. Conclusion Adoptive transfer of dendritic cells pulsed with apoptotic tumor cells as a source of tumor antigens, can elicit effective antitumor responses in the poorly immunogenic SCC model when combined with interleukin‐2. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1097/00005537-200108000-00026 |
| الإتاحة: |
https://doi.org/10.1097/00005537-200108000-00026Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.6D25A807 |
| قاعدة البيانات: |
BASE |
