Molecular characterization of pyrethroid knockdown resistance (kdr ) in the major malaria vector Anopheles gambiae s.s
| العنوان: | Molecular characterization of pyrethroid knockdown resistance (kdr ) in the major malaria vector Anopheles gambiae s.s |
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| المؤلفون: | David Martínez-Torres, Alan L. Devonshire, Nicole Pasteur, Fabrice Chandre, Frédéric Darriet, Martin S. Williamson, Jean Baptiste Berge, David Pauron, Pierre Guillet |
| المساهمون: | ProdInra, Migration, INRA, Génétique moléculaire des Invertébrés, Institut National de la Recherche Agronomique (INRA), Laboratoire de Lutte Contre les Insectes Nuisibles (LIN), Department of Biological and Ecological Chemistry, IACR-Rothamsted, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie des invertébrés |
| المصدر: | Insect Molecular Biology Insect Molecular Biology, Wiley, 1998, 7 (2), pp.179-184. ⟨10.1046/j.1365-2583.1998.72062.x⟩ Insect Molecular Biology, Wiley, 1998, 7 (2), pp.179-184 |
| بيانات النشر: | Wiley |
| مصطلحات موضوعية: | Anopheles gambiae, Sodium channel gene, [SDV]Life Sciences [q-bio], diagnostic, medicine.disease_cause, Sodium Channels, Insecticide Resistance, chemistry.chemical_compound, 0302 clinical medicine, DIAGNOSTIC, Pyrethrins, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Mutation, Pyrethroid, PYRETHROIDES RESISTANCE, biology, pyrethroid, 3. Good health, [SDV] Life Sciences [q-bio], MOUSTIQUE, LUTTE ANTIVECTORIELLE, Insect Proteins, Female, Biochemistry & Molecular Biology, DNA, Complementary, 030231 tropical medicine, Molecular Sequence Data, Bendiocarb, mosquito, resistance, 03 medical and health sciences, MALARIA, Anopheles, parasitic diseases, Genetics, medicine, [SDE.BE.EVO]Environmental Sciences/Biodiversity and Ecology/domain_sde.be.evo, Animals, Amino Acid Sequence, Molecular Biology, Alleles, 030304 developmental biology, mosquitoes, Base Sequence, business.industry, Diptera, VECTEUR, Knockdown resistance, Sequence Analysis, DNA, PALUDISME, biology.organism_classification, medicine.disease, Virology, Biotechnology, Insect Vectors, Culicidae, chemistry, Insect Science, Vector (epidemiology), sodium-channel gene, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business, MOUSTIQUAIRE IMPREGNEE, Entomology, Malaria, RESISTANCE |
| الوصف: | International audience; Pyrethroid-impregnated bednets are playing an increasing role for combating malaria, especially in stable malaria areas. More than 90% of the current annual malaria incidence (c. 500 million clinical cases with up to 2 million deaths) is in Africa where the major vector is Anopheles gambiae s.s. As pyrethroid resistance has been reported in this mosquito, reliable and simple techniques are urgently needed to characterize and monitor this resistance in the field, In insects, an important mechanism of pyrethroid resistance is due to a modification of the voltage-gated sodium channel protein recently shown to be associated with mutations of the para-type sodium channel gene. We demonstrate here that one of these mutations is present in certain strains of pyrethroid resistant A. gambiae s.s. and describe a PCR-based diagnostic test allowing its detection in the genome of single mosquitoes, Using this test, we found this mutation in six out of seven field samples from West Africa, its frequency being closely correlated with survival to pyrethroid exposure. This diagnostic test should bring major improvement for field monitoring of pyrethroid resistance, within the framework of malaria control programmes. |
| وصف الملف: | application/pdf; text/pdf |
| تدمد: | 0962-1075 1365-2583 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::844d734ee158979f07217fe5cb49068fTest https://doi.org/10.1046/j.1365-2583.1998.72062.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....844d734ee158979f07217fe5cb49068f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09621075 13652583 |
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