Effect of stationary magnetic field strengths of 150 and 200 mT on reactive oxygen species production in soybean

التفاصيل البيبلوغرافية
العنوان: Effect of stationary magnetic field strengths of 150 and 200 mT on reactive oxygen species production in soybean
المؤلفون: Anjali Anand, M.B. Shine, K.N. Guruprasad
المصدر: Bioelectromagnetics. 33:428-437
بيانات النشر: Wiley, 2012.
سنة النشر: 2012
مصطلحات موضوعية: Time Factors, Antioxidant, Physiology, medicine.medical_treatment, Radical, Biophysics, Germination, Ascorbic Acid, Antioxidants, Superoxide dismutase, chemistry.chemical_compound, medicine, Radiology, Nuclear Medicine and imaging, Hydrogen peroxide, chemistry.chemical_classification, Reactive oxygen species, biology, Superoxide, Electron Spin Resonance Spectroscopy, food and beverages, Organ Size, General Medicine, Ascorbic acid, Magnetic Fields, Biochemistry, chemistry, Seedlings, Seeds, biology.protein, Soybeans, Reactive Oxygen Species, Peroxidase
الوصف: Our previous investigation reported the beneficial effect of pre-sowing magnetic treatment for improving germination parameters and biomass accumulation in soybean. In this study, soybean seeds treated with static magnetic fields of 150 and 200 mT for 1 h were evaluated for reactive oxygen species (ROS) and activity of antioxidant enzymes. Superoxide and hydroxyl radicals were measured in embryos and hypocotyls of germinating seeds by electron paramagnetic resonance spectroscopy and kinetics of superoxide production; hydrogen peroxide and antioxidant activities were estimated spectrophotometrically. Magnetic field treatment resulted in enhanced production of ROS mediated by cell wall peroxidase while ascorbic acid content, superoxide dismutase and ascorbate peroxidase activity decreased in the hypocotyl of germinating seeds. An increase in the cytosolic peroxidase activity indicated that this antioxidant enzyme had a vital role in scavenging the increased H2O2 produced in seedlings from the magnetically treated seeds. Hence, these studies contribute to our first report on the biochemical basis of enhanced germination and seedling growth in magnetically treated seeds of soybean in relation to increased production of ROS. Bioelectromagnetics 33:428–437, 2012. © 2012 Wiley Periodicals, Inc.
تدمد: 0197-8462
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cc357db7058b5a859ecf9290a7c4b38Test
https://doi.org/10.1002/bem.21702Test
حقوق: CLOSED
رقم الانضمام: edsair.doi.dedup.....3cc357db7058b5a859ecf9290a7c4b38
قاعدة البيانات: OpenAIRE