التفاصيل البيبلوغرافية
| العنوان: |
Disruption of Staphylococcus epidermidis biofilm formation using a targeted cationic peptide |
| المؤلفون: |
Christopher M. Hofmann, Kyle J. Bednar, Roger E. Marchant, James M. Anderson |
| المصدر: |
Journal of Biomedical Materials Research Part A. :1061-1067 |
| بيانات النشر: |
Wiley, 2012. |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
Molecular Sequence Data, Biomedical Engineering, Virulence, Peptide, Microbiology, Biomaterials, chemistry.chemical_compound, Staphylococcus epidermidis, Cations, Amino Acid Sequence, Fluorescent Dyes, chemistry.chemical_classification, biology, Metals and Alloys, Biofilm, biology.organism_classification, Wheat germ agglutinin, chemistry, Biochemistry, Biofilms, Polylysine, Microscopy, Electron, Scanning, Ceramics and Composites, Nucleic acid, Peptides, Bacteria |
| الوصف: |
This study reports the use of a targeted cationic peptide with the ability to disrupt Staphylococcus epidermidis biofilm formation. Complications due to nosocomial infections of implanted medical devices pose a significant health risk to patients, with Staphylococcus epidermidis often implicated in the case of blood-contacting biomaterials. S. epidermidis virulence relies mainly on its ability to form a biofilm, the main component of which is polysaccharide intercellular adhesin (PIA). We utilized the synthetic β6-20 peptide, known to specifically bind S. epidermidis, in order to deliver a cationic polylysine peptide (G3K6) to the bacterial surface and disrupt the charge–charge interactions needed for PIA retention and biofilm stability. The effects of the β6-20-G3K6 peptide on biofilm formation were assessed using optical density, fluorescently labeled wheat germ agglutinin, nucleic acid stain (SYTO 9), and a metabolic assay (XTT, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt). Biofilms formed in the presence of β6-20-G3K6 peptide (100 μM) resulted in a 37.9% reduction in PIA content and a 17.5% reduction of adherent bacteria relative to biofilms grown in the absence of peptide. These studies demonstrate the targeting ability of the β6-20 peptide towards biomaterial-adherent S.epidermidis, and highlight the potential for disrupting the early stages of biofilm formation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012. |
| تدمد: |
1549-3296 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdf1f0cbea0ed8594a97e07263348648Test
https://doi.org/10.1002/jbm.a.33273Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi.dedup.....cdf1f0cbea0ed8594a97e07263348648 |
| قاعدة البيانات: |
OpenAIRE |
