Detection of Minimal Residual Disease in B Cell Acute Lymphoblastic Leukemia Using an Eight‐Color Tube with Dried Antibody Reagents
| العنوان: | Detection of Minimal Residual Disease in B Cell Acute Lymphoblastic Leukemia Using an Eight‐Color Tube with Dried Antibody Reagents |
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| المؤلفون: | Isabelle Arnoux, Lakhdar Bouriche, Marie Loosveld, Vanessa Nivaggioni, Denis Bernot |
| المساهمون: | Laboratoire d'hématologie biologique [Hôpital de la Timone - Hôpital Nord - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Fibrinolyse et Pathologie Vasculaire, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition (C2VN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre interdisciplinaire de recherche en biologie (CIRB), Collège de France (CdF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-PSL Research University (PSL)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | CYTOMETRY PART B-CLINICAL CYTOMETRY CYTOMETRY PART B-CLINICAL CYTOMETRY, 2019, 96 (2), pp.158-163. ⟨10.1002/cyto.b.21766⟩ |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Quality Control, 0301 basic medicine, Neoplasm, Residual, Histology, Adolescent, Antibodies, Pathology and Forensic Medicine, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, hemic and lymphatic diseases, medicine, Humans, Tube (fluid conveyance), Child, B cell, biology, medicine.diagnostic_test, business.industry, Infant, Cell Biology, Flow Cytometry, Minimal residual disease, 3. Good health, Staining, 030104 developmental biology, medicine.anatomical_structure, Child, Preschool, 030220 oncology & carcinogenesis, Acute Disease, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Indicators and Reagents, Bone marrow, Antibody, Nuclear medicine, business, Cytometry |
| الوصف: | International audience; Background Flow cytometry is a powerful tool for the detection of minimal residual disease (MRD) of B cell precursor acute lymphoblastic leukemia (BCP-ALL) patients. However, the staining process and the choice of antibodies rely on laboratory expertise and may be source of variability or technical errors. Recently, Beckman Coulter commercialized a ready to use tube with dried format reagents for BCP-ALL MRD detection. The aim of this study is to evaluate the applicability of this tube and to compare it to a conventional (liquid format reagents) method. Methods Thirty-one samples from B ALL patients were analyzed: 19 bone marrow (BM) aspirations, 10 peripheral blood (PB) samples and 2 cerebrospinal fluids at different stages of the follow-up. In addition, we tested 5 bone marrow samples mixed into non-pathological (control) bone marrow. The dried format tube included seven antibodies: CD45Kro, CD58FITC, CD34ECD, CD10PC5.5, CD19PC7, CD38AA700, CD20AA750, with possibility of additional antibodies for blast markers identified at diagnosis. For comparison, a liquid format tube was prepared, and considered as the reference. Results This tube was validated for daily routine laboratory, with satisfying qualitative (MRD + or MRD-) and quantitative (MRD percentages) correlation with the reference tube. Conclusion With this single dried format tube, we showed interesting results for BCP-ALL MRD detection in the aim of standardization and reliable interlaboratory results. It allows accurate MRD detection including low levels (10-4), and offers possibility to increase performance (supplementary antibody) within a preestablished effective antibody panel for BCP-ALL MRD. (c) 2018 International Clinical Cytometry Society |
| تدمد: | 1552-4957 1552-4949 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd04bdfcdaef20ff60396a7f442d207eTest https://doi.org/10.1002/cyto.b.21766Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fd04bdfcdaef20ff60396a7f442d207e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15524957 15524949 |
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