التفاصيل البيبلوغرافية
| العنوان: |
Changes in expression of markers of GABA transmission in nucleus of the solitary tract (NTS) of ovariectomized (OVX) female rats |
| المؤلفون: |
Spary, Emma Jane, Maqbool, Azhar, Drinkhill, Mark J, Batten, Trevor FC |
| المصدر: |
The FASEB Journal ; volume 26, issue S1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2012 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Estrogen is a modulator of autonomic function and we previously showed differences in estrogen receptor expression in NTS associated with plasma estrogen levels during the estrous cycle in female rats. As promoter sequences for several receptors, receptor associated proteins, enzymes and transporters involved in GABA transmission include potential estrogen response element consensus sites we analyzed levels of mRNA expression by real time PCR in NTS micropunches from control cycling females and 3 week OVX rats. Plasma estradiol levels were measured by ELISA and blood pressures recorded via implanted telemetry probes. For 6 of the GABA‐A subunits analyzed (alpha1; alpha3; beta1; gamma1, gamma3, delta) there were significant reductions in mRNA expression in OVX compared to rats in either the low estrogen (metestrus) or high estrogen (proestrus) phases of the cycle (p<0.05; ANOVA; n = 6). Expression of the GABA‐B receptor B1 subtype showed a highly significant reduction (p<0.01) in OVX, as did the expression levels for GABARAP and GABARAPL1/2. Differences (p<0.05) between the metestrus and OVX expression levels were found for GAD65, GAD67 and vGAT. The data suggest that circulating estrogen acts in NTS to modulate inhibitory GABAergic influences on autonomic reflexes through affecting both the GABA release mechanism and GABA receptor composition. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.26.1_supplement.1091.56 |
| الإتاحة: |
https://doi.org/10.1096/fasebj.26.1_supplement.1091.56Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.81760BBC |
| قاعدة البيانات: |
BASE |
