Purpose To explore the feasibility of T1rho mapping of menisci at 3T in discriminating between patients with acute anterior cruciate ligament (ACL) injury and healthy controls. Materials and Methods Thirty-three subjects were included in the study and subdivided into two subgroups: 16 healthy controls (4 females, 12 males; mean age = 34.4 ± 10.2 years, age range 24–63 years), 17 patients with ACL injury (3 females, 14 males; mean age = 29.8 ± 10.8 years, age range 18–61 years). T1rho images from all subjects were acquired on a 3T MR scanner using a spin-lock-based 3D GRE sequence and computed for T1rho mapping. Clinical proton density (PD)-weighted fast spin echo (FSE) images in the sagittal (without fat saturation), axial, and coronal (fat-saturated) planes were also acquired for cartilage assessment using Whole-Organ MR Imaging Score (WORMS) grading. Mixed model two-way analysis of variance (ANOVA) was performed to determine whether there were any significant differences among subregional, compartmental, and whole structure T1rho values of meniscus between healthy controls and ACL-injured patients. Results Lateral posterior (29 ± 8 msec) and medial central (25 ± 7 msec) meniscus subregions in healthy controls had significantly lower T1rho values (P