Cellular responses of NG108-15 and SK-N-MC lines to sweet and bitter tastants as measured by extracellular acidification rates
| العنوان: | Cellular responses of NG108-15 and SK-N-MC lines to sweet and bitter tastants as measured by extracellular acidification rates |
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| المؤلفون: | Kuppan Gokulan, Sangeeta Khare, Darwin S. Linthicum |
| المصدر: | Journal of Neuroscience Research. 63:64-71 |
| بيانات النشر: | Wiley, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Taste, chemistry.chemical_element, Receptors, Cell Surface, Calcium, Cellular and Molecular Neuroscience, Tissue culture, chemistry.chemical_compound, GTP-Binding Proteins, Tumor Cells, Cultured, Extracellular, Animals, Humans, Receptor, Dose-Response Relationship, Drug, Quinine, Chemistry, Denatonium, food and beverages, Analgesics, Non-Narcotic, Biochemistry, Cell culture, Sweetening Agents, Signal transduction, Extracellular Space, Acids, Signal Transduction |
| الوصف: | The Cytosensor microphysiometer device (Molecular Devices, Sunnyvale, CA) is capable of detecting small changes in cellular metabolism in response to specific bioactive ligands by measuring the extracellular acidification rate (ECAR). By measuring the ECAR we were able to detect responses of tissue culture cell lines to a variety of sweet- and bitter-tasting compounds. We examined in detail the responses of the NG108-15 (mouse neuroblastoma x rat glioma hybrid) and SK-N-MC (human neuroepithelioma) cell lines. We determined that NG108-15 cells were consistently very responsive to several potent sweeteners and bitter compounds, such as sodium saccharin, guanidino- sweeteners, denatonium benzoate, quinine, and ranitidine. These compounds could evoke changes in cellular metabolism (measured as ECAR) that were rapid in onset, saturable with respect to ligand concentration, and sensitive to several inhibitors of G-protein-coupled receptor signaling pathways. In sharp contrast, the neuroepithelioma SK-N-MC did not respond to any of the sweet or bitter compounds. Rapid changes in ECAR were easily detectable in both cell lines with the calcium ionophore A23187. Bradykinin elicited changes in the ECAR only in the NG108-15 cell line, which is known to express the B2 receptor. The changes in ECAR of the NG108-15 cell line in response to sweet and bitter taste compounds suggest these cells may expresses a receptor(s) specific for small sapid molecules. |
| تدمد: | 1097-4547 0360-4012 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff9393c236cee0358246275df995ec7fTest https://doi.org/10.1002/1097-4547Test(20010101)63:1<64::aid-jnr8>3.0.co;2-t |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ff9393c236cee0358246275df995ec7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974547 03604012 |
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