دورية أكاديمية
Evaluation of an enzyme immunoassay and immunodiffusion for detection of anti‐Histoplasma antibodies in serum from cats and dogs
| العنوان: | Evaluation of an enzyme immunoassay and immunodiffusion for detection of anti‐Histoplasma antibodies in serum from cats and dogs |
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| المؤلفون: | Rebecca Tims, Andrew S. Hanzlicek, Laura Nafe, Michelle M. Durkin, Jennifer Smith‐Davis, L. Joseph Wheat |
| المصدر: | Journal of Veterinary Internal Medicine, Vol 37, Iss 3, Pp 1007-1014 (2023) |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | canine, feline, fungal infection, histoplasmosis, invasive fungal infection, serology, Veterinary medicine, SF600-1100 |
| الوصف: | Abstract Background Histoplasma antigen and anti‐Histoplasma antibody detection are used to support the diagnosis of histoplasmosis. There is a paucity of published data on antibody assays. Objectives Our primary hypothesis was that anti‐Histoplasma immunoglobulin G (IgG) antibody detection using enzyme immunoassay (EIA) will be more sensitive as compared to immunodiffusion (ID). Animals Thirty‐seven cats and 22 dogs with proven or probable histoplasmosis; 157 negative control animals. Methods Residual stored sera were tested for anti‐Histoplasma antibodies using EIA and ID. Results of urine antigen EIA were reviewed retrospectively. Diagnostic sensitivity was calculated for all three assays and compared between immunoglobulin G (IgG) EIA and ID. The diagnostic sensitivity of urine antigen EIA and IgG EIA, interpreted in parallel, was reported. Results Sensitivity of IgG EIA was 30/37 (81.1%; 95% confidence interval [CI], 68.5%‐93.4%) in cats and 17/22 (77.3%; 95% CI, 59.8%‐94.8%) in dogs. Diagnostic sensitivity of ID was 0/37 (0%; 95% CI, 0%‐9.5%) in cats and 3/22 (13.6%; 95% CI, 0%‐28.0%) in dogs. Immunoglobulin G EIA was positive in all animals (2 cats and 2 dogs) with histoplasmosis but without detectable antigen in urine. Diagnostic specificity of IgG EIA was 18/19 (94.7%; 95% CI, 74.0%‐99.9%) in cats and 128/138 (92.8%; 95% CI, 87.1%‐96.5%) in dogs. Conclusion and Clinical Importance Antibody detection by EIA can be used to support the diagnosis of histoplasmosis in cats and dogs. Immunodiffusion has an unacceptably low diagnostic sensitivity and is not recommended. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1939-1676 0891-6640 |
| العلاقة: | https://doaj.org/toc/0891-6640Test; https://doaj.org/toc/1939-1676Test |
| DOI: | 10.1111/jvim.16726 |
| الوصول الحر: | https://doaj.org/article/d2ee80b26a9b4691bfab94dbc754b57fTest |
| رقم الانضمام: | edsdoj.2ee80b26a9b4691bfab94dbc754b57f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19391676 08916640 |
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| DOI: | 10.1111/jvim.16726 |