HIV-1 Tat protein concomitantly down-regulates apical caspase-10 and up-regulates c-FLIP in lymphoid T cells: A potential molecular mechanism to escape TRAIL cytotoxicity
| العنوان: | HIV-1 Tat protein concomitantly down-regulates apical caspase-10 and up-regulates c-FLIP in lymphoid T cells: A potential molecular mechanism to escape TRAIL cytotoxicity |
|---|---|
| المؤلفون: | Davide Gibellini, Giorgio Zauli, Greta Fabbri, Cristina Ponti, Maria Grazia di Iasio, Francesca Vitone, Isabella Bon, Maria Carla Re |
| المساهمون: | Gibellini, D, CARLA RE, M, Ponti, Cristina, Vitone, F, Bon, I, Fabbri, G, DI IASIO, Mg, Zauli, G., GIBELLINI D, RE M., PONTI C, VITONE F, BON I, FABBRI G, DI IASIO G, ZAULI G |
| المصدر: | Journal of Cellular Physiology. 203:547-556 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, DNA, Complementary, c-FLIP, Cell Survival, Physiology, T cell, Clinical Biochemistry, caspase-10, CASP8 and FADD-Like Apoptosis Regulating Protein, Down-Regulation, Apoptosis, HIV Infections, TRAIL, Biology, Jurkat cells, Tat protein, TNF-Related Apoptosis-Inducing Ligand, Jurkat Cells, medicine, Humans, RNA, Messenger, Caspase 10, Cytotoxicity, HIV-1, caspase-10, TRAIL, c-flip, Tat, Caspase 8, Messenger RNA, Membrane Glycoproteins, HIV-1, caspases, Tumor Necrosis Factor-alpha, Lymphoblast, Antibody-Dependent Cell Cytotoxicity, Intracellular Signaling Peptides and Proteins, Cell Biology, Transfection, Molecular biology, Up-Regulation, medicine.anatomical_structure, Caspases, Gene Products, tat, Mutation, RNA, Viral, tat Gene Products, Human Immunodeficiency Virus, Apoptosis Regulatory Proteins |
| الوصف: | In this study, we showed the existence of a positive correlation between the amount of human immunodeficiency virus-type 1 (HIV-1) RNA in HIV-1 seropositive subjects and the plasma levels of TRAIL. Since it has been previously demonstrated that HIV-1 Tat protein up-regulates the expression of TRAIL in monocytic cells whereas tat-expressing lymphoid cells are more resistant to TRAIL cytotoxicity, we next investigated the effect of Tat on the expression/activity of both apical caspase-8 and -10, which play a key role in mediating the initial phases of apoptosis by TRAIL, and c-FLIP. Jurkat lymphoblastoid human T cell lines stably transfected with a plasmid expressing wild-type (HIV-1) tat gene showed normal levels of caspase-8 but significantly decreased levels of caspase-10 at both mRNA and protein levels with respect to Jurkat transfected with the control plasmid or with a mutated (cys22) non-functional tat cDNA. A significant decrease of caspase-10 expression/activity was also observed in transient transfection experiments with plasmid carrying tat cDNA. Moreover, c-FLIP(L) and c-FLIP(S) isoforms were up-regulated in tat-expressing cells at both mRNA and protein level in comparison with control cells. Taken together, these results provide a molecular basis to explain the resistance of tat-expressing Jurkat cells to apoptosis induced by TRAIL and, possibly, to other death-inducing ligands. Copyright 2004 Wiley-Liss, Inc. |
| وصف الملف: | STAMPA |
| تدمد: | 1097-4652 0021-9541 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e04a421202468e654d79c8f6d0072b59Test https://doi.org/10.1002/jcp.20252Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e04a421202468e654d79c8f6d0072b59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
|---|