Differential expression of miRNAs and their target genes in senescing leaves and siliques: insights from deep sequencing of small RNAs and cleaved target RNAs
| العنوان: | Differential expression of |
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| المؤلفون: | Shawn R. Thatcher, Amnon Lers, Pamela J. Green, Christopher D Wright, Shaul Burd |
| المصدر: | Plant, Cell & Environment |
| بيانات النشر: | Wiley, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | 0106 biological sciences, Senescence, Small RNA, Time Factors, senescence, Physiology, nutrient remobilization, Arabidopsis, Plant Science, Biology, 01 natural sciences, Deep sequencing, 03 medical and health sciences, Gene Expression Regulation, Plant, microRNA, RNA, Messenger, PARE, Gene, Cellular Senescence, A rabidopsis thaliana, Gene Library, 030304 developmental biology, Genetics, 0303 health sciences, Sequence Analysis, RNA, High-Throughput Nucleotide Sequencing, RNA, Original Articles, silique, biology.organism_classification, Cell biology, Plant Leaves, MicroRNAs, RNA, Plant, Cell aging, 010606 plant biology & botany |
| الوصف: | MicroRNAs (miRNAs) are a class of small RNAs, which typically function by guiding cleavage of target mRNAs. They are known to play roles in a variety of plant processes including development, responses to environmental stresses and senescence. To identify senescence regulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel analysis of RNA ends libraries, which enable the large-scale examination of miRNA-guided cleavage products, were constructed and sequenced, resulting in over 750 million genome-matched sequences. These large datasets led to the identification a new senescence-inducible small RNA locus, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. |
| تدمد: | 1365-3040 0140-7791 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43fa0a7047b87994666bbcee8cd6638eTest https://doi.org/10.1111/pce.12393Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....43fa0a7047b87994666bbcee8cd6638e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13653040 01407791 |
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