Autophagy machinery controls nitrogen remobilization at the whole‐plant level under both limiting and ample nitrate conditions in Arabidopsis
| العنوان: | Autophagy machinery controls nitrogen remobilization at the whole‐plant level under both limiting and ample nitrate conditions in Arabidopsis |
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| المؤلفون: | Céline Masclaux-Daubresse, Fabienne Soulay, Anne Guiboileau, Marie-Paule Bataillé, Jean-Christophe Avice, Kohki Yoshimoto |
| المساهمون: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), SAKURA [21124QA], French Ministere des Affaires Etrangeres et Europeennes, Japan Society for the Promotion of Science, Institut Jean-Pierre Bourgin ( IJPB ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Ecophysiologie Végétale, Agronomie et Nutritions ( EVA ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Recherche Agronomique ( INRA ) |
| المصدر: | New Phytologist New Phytologist, Wiley, 2012, 194 (3), pp.732-740. ⟨10.1111/j.1469-8137.2012.04084.x⟩ New Phytologist, Wiley, 2012, 194 (3), pp.732-740. 〈10.1111/j.1469-8137.2012.04084.x〉 |
| بيانات النشر: | Wiley, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0106 biological sciences, Physiology, Mutant, Arabidopsis, NATURAL VARIATION, Plant Science, Vacuole, 01 natural sciences, Autophagy-Related Protein 5, chemistry.chemical_compound, Nitrate, CHLOROPLASTS, Biomass, [ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences, harvest index (HI), Cellular Senescence, 2. Zero hunger, 0303 health sciences, biology, 15N, food and beverages, nitrogen remobilization, Plants, Genetically Modified, Chloroplast, Phenotype, Seeds, RNA Interference, RIBULOSE-1, autophagy, Nitrogen, PROTEINS, Ribulose-Bisphosphate Carboxylase, 03 medical and health sciences, Botany, LEAVES, LEAF SENESCENCE, Nitrogen cycle, 030304 developmental biology, Nitrates, Nitrogen Isotopes, THALIANA, Arabidopsis Proteins, 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE, Autophagy, RuBisCO, DEGRADATION, yield, biology.organism_classification, RUBISCO, Carbon, Phosphoric Monoester Hydrolases, Plant Leaves, chemistry, Mutation, Vacuoles, biology.protein, nitrogen use efficiency (NUE), STARVATION, 010606 plant biology & botany |
| الوصف: | • Processes allowing the recycling of organic nitrogen and export to young leaves and seeds are important determinants of plant yield, especially when plants are nitrate-limited. Because autophagy is induced during leaf ageing and in response to nitrogen starvation, its role in nitrogen remobilization was suspected. It was recently shown that autophagy participates in the trafficking of Rubisco-containing bodies to the vacuole. • To investigate the role of autophagy in nitrogen remobilization, several autophagy-defective (atg) Arabidopsis mutants were grown under low and high nitrate supplies and labeled with at the vegetative stage in order to determine (15) N partitioning in seeds at harvest. Because atg mutants displayed earlier and more rapid leaf senescence than wild type, we investigated whether their defects in nitrogen remobilization were related to premature leaf cell death by studying the stay-green atg5.sid2 and atg5.NahG mutants. • Results showed that nitrogen remobilization efficiency was significantly lower in all the atg mutants irrespective of biomass defects, harvest index reduction, leaf senescence phenotypes and nitrogen conditions. • We conclude that autophagy core machinery is needed for nitrogen remobilization and seed filling. |
| تدمد: | 1469-8137 0028-646X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16e0d13c76f3b210a658986c99632de7Test https://doi.org/10.1111/j.1469-8137.2012.04084.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....16e0d13c76f3b210a658986c99632de7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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