Imaging of endodontic biofilms by combined microscopy (FISH/cLSM - SEM)
| العنوان: | Imaging of endodontic biofilms by combined microscopy (FISH/cLSM - SEM) |
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| المؤلفون: | Christoph Schaudinn, Gary B. Carr, David E. Jaramillo, Amita Gorur, Paul Webster, John William Costerton |
| المصدر: | Journal of Microscopy. 235:124-127 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Microscopy, Confocal, Histology, Materials science, Bacteria, medicine.diagnostic_test, Scanning electron microscope, Resolution (electron density), Scanning confocal electron microscopy, Biofilm, Nanotechnology, Pathology and Forensic Medicine, Biofilms, Microscopy, Image Processing, Computer-Assisted, Microscopy, Electron, Scanning, medicine, Scanning ion-conductance microscopy, Humans, Fish |
| الوصف: | Scanning electron microscopy is a useful imaging approach for the visualization of bacterial biofilms in their natural environments including their medical and dental habitats, because it allows for the exploration of large surfaces with excellent resolution of topographic features. Most biofilms in nature, however, are embedded in a thick layer of extracellular matrix that prevents a clear identification of individual bacteria by scanning electron microscopy. The use of confocal laser scanning microscopy on the other hand in combination with fluorescence in situ hybridization enables the visualization of matrix embedded bacteria in multi-layered biofilms. In our study, fluorescence in situ hybridization/confocal laser scanning microscopy and scanning electron microscopy were applied to visualize bacterial biofilm in endodontic root canals. The resulting fluorescence in situ hybridization /confocal laser scanning microscopy and scanning electron microscopy and pictures were subsequently combined into one single image to provide high-resolution information on the location of hidden bacteria. The combined use of scanning electron microscopy and fluorescence in situ hybridization / confocal laser scanning microscopy has the potential to overcome the limits of each single technique. |
| تدمد: | 1365-2818 0022-2720 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::209c57724d85801d06e137d2b6cefe39Test https://doi.org/10.1111/j.1365-2818.2009.03201.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....209c57724d85801d06e137d2b6cefe39 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652818 00222720 |
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