Alternative mRNA Splicing of the Novel GTPase Rab28 Generates Isoforms with Different C-Termini
| العنوان: | Alternative mRNA Splicing of the Novel GTPase Rab28 Generates Isoforms with Different C-Termini |
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| المؤلفون: | Silke Massmann, Walter Becker, Hans-Georg Joost, Chichung Lie, Andreas Brauers, Heikki Kainulainen, Annette Schürmann, Petra Mühl‐zürbes |
| المصدر: | European Journal of Biochemistry. 237:833-840 |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Male, Gene isoform, DNA, Complementary, Molecular Sequence Data, GTPase, Biology, Biochemistry, GTP Phosphohydrolases, Species Specificity, GTP-Binding Proteins, Complementary DNA, Animals, Humans, Coding region, Tissue Distribution, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Peptide sequence, chemistry.chemical_classification, Base Sequence, Sequence Homology, Amino Acid, cDNA library, Alternative splicing, Brain, Molecular biology, Recombinant Proteins, Rats, Amino acid, Isoenzymes, Alternative Splicing, Adipose Tissue, chemistry, rab GTP-Binding Proteins |
| الوصف: | A novel ras-related gene (rab28) was identified by a PCR-based cloning approach and subsequent screening of rat fat cell and brain cDNA libraries. The deduced amino acid sequence of the cDNA is distantly related with members of the Rab family (31-33% sequence identity, mainly restricted to the six GTP-binding motifs). Cloning of the human homologue of Rab28 by a PCR-based approach revealed the existence of two isoforms (hRab28S, hRab28L) which differ only by a 95-bp insertion within the coding region. This insertion generates an alternative sequence of the 30 C-terminal amino acids of the protein. Both C-termini of the human homologues comprise farnesylation motifs, but differ strikingly in a stretch of 13 amino acids. By PCR, mRNA of hRab28S was detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas hRab28L was predominant in testis. Recombinant Rab28 proteins showed specific binding of radiolabeled guanosine 5'-O-[gamma-thio]triphosphate and rapidly hydrolysed [alpha-32P]GTP; there was no difference in the GTP binding characteristics of the two isoforms hRab28S and hRab28L. It is suggested that the isoforms are derived from the same gene by alternative mRNA splicing, and that their functions differ in a parameter unrelated to its basic role as a GTPase. |
| تدمد: | 1432-1033 0014-2956 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7f7b1bd57139fdb67374727e636d0b5Test https://doi.org/10.1111/j.1432-1033.1996.0833p.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d7f7b1bd57139fdb67374727e636d0b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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