Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas
| العنوان: | Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas |
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| المؤلفون: | Karim Bouzakri, Eric Delpy, Elisa Maillard, Franck Zal, Séverine Sigrist, Florent Lemaire, C. Peronet, Julien Cherfan, Michel Pinget |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Time Factors, Necrosis, Islets of Langerhans Transplantation, medicine.disease_cause, p38 Mitogen-Activated Protein Kinases, necrosis, 0302 clinical medicine, Insulin Secretion, islet isolation, oxidative stress, HMGB1 Protein, Kidney transplantation, geography.geographical_feature_category, Chemistry, Cold Ischemia, Organ Preservation, Islet, medicine.anatomical_structure, oxygen carrier, 030220 oncology & carcinogenesis, Molecular Medicine, Original Article, medicine.symptom, Pancreas, pancreas preservation, endocrine system, medicine.medical_specialty, Cell Survival, Organ Preservation Solutions, Ischemia, Islets of Langerhans, 03 medical and health sciences, Internal medicine, medicine, Animals, Humans, Rats, Wistar, Protein kinase B, geography, Electron Transport Complex I, cold ischaemia, islet transplantation, Original Articles, Cell Biology, medicine.disease, Rats, Oxygen, Transplantation, 030104 developmental biology, Endocrinology, Reactive Oxygen Species, Proto-Oncogene Proteins c-akt, Oxidative stress |
| الوصف: | Ischaemia impairs organ quality during preservation in a time‐dependent manner, due to a lack of oxygen supply. Its impact on pancreas and islet transplantation outcome has been demonstrated by a correlation between cold ischaemia time and poor islet isolation efficiency. Our goal in the present study was to improve pancreas and islet quality using a novel natural oxygen carrier (M101, 2 g/L), which has been proven safe and efficient in other clinical applications, including kidney transplantation, and for several pre‐clinical transplantation models. When M101 was added to the preservation solution of rat pancreas during ischaemia, a decrease in oxidative stress (ROS), necrosis (HMGB1), and cellular stress pathway (p38 MAPK)activity was observed. Freshly isolated islets had improved function when M101 was injected in the pancreas. Additionally, human pancreases exposed to M101 for 3 hours had an increase in complex 1 mitochondrial activity, as well as activation of AKT activity, a cell survival marker. Insulin secretion was also up‐regulated for isolated islets. In summary, these results demonstrate a positive effect of the oxygen carrier M101 on rat and human pancreas during preservation, with an overall improvement in post‐isolation islet quality. |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7b17cc156a8d7fec1e3977501011800Test https://doi.org/10.1111/jcmm.14666Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c7b17cc156a8d7fec1e3977501011800 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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