تقرير
Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70.
| العنوان: | Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70. |
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| المؤلفون: | Ciesielski, Szymon J, Schilke, Brenda A, Stolarska, Milena, Tonelli, Marco, Tomiczek, Bartlomiej, Craig, Elizabeth A |
| المصدر: | FEBS Lett ; ISSN:1873-3468 ; Volume:598 ; Issue:12 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Hsp40, JDP, J‐domain, heat shock protein, molecular chaperone, protein homeostasis |
| الوصف: | J-domain proteins are critical Hsp70 co-chaperones. A and B types have a poorly understood glycine-rich region (Grich) adjacent to their N-terminal J-domain (Jdom). We analyzed the ability of Jdom/Grich segments of yeast Class B Sis1 and a suppressor variant of Class A, Ydj1, to rescue the inviability of sis1-∆. In each, we identified a cluster of Grich residues required for rescue. Both contain conserved hydrophobic and acidic residues and are predicted to form helices. While, as expected, the Sis1 segment docks on its J-domain, that of Ydj1 does not. However, data suggest both interact with Hsp70. We speculate that the Grich-Hsp70 interaction of Classes A and B J-domain proteins can fine tune the activity of Hsp70, thus being particularly important for the function of Class B. |
| نوع الوثيقة: | report article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1002/1873-3468.14857Test; https://pubmed.ncbi.nlm.nih.gov/38529663Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11209805Test/ |
| DOI: | 10.1002/1873-3468.14857 |
| الإتاحة: | https://doi.org/10.1002/1873-3468.14857Test https://pubmed.ncbi.nlm.nih.gov/38529663Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11209805Test/ |
| حقوق: | © 2024 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. |
| رقم الانضمام: | edsbas.60AA2357 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/1873-3468.14857 |
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