مورد إلكتروني
Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes
| العنوان: | Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes |
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| بيانات النشر: | KTH, Biomedicinsk fysik och röntgenfysik KTH, Fysik Wiley 2021 |
| تفاصيل مُضافة: | Romell, Jenny Jie, V. W. Miettinen, A. Baird, E. Hertz, Hans |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done. QC 20220301 |
| مصطلحات الفهرس: | bumblebee, compound eyes, laboratory nano-CT, phase contrast, system comparison, glucose, glutaraldehyde, nanotube, osmium tetraoxide, paraformaldehyde, phosphotungstic acid, acceleration, Article, basement membrane, Bombus terrestris, comparative study, compound eye, controlled study, electric field, electron microscopy, histology, illumination, image processing, micro-computed tomography, nonhuman, phase contrast microscopy, photoreceptor cell, reconstruction algorithm, signal noise ratio, transmission electron microscopy, animal, bee, devices, laboratory, procedures, synchrotron, x-ray computed tomography, Animals, Bees, Laboratories, Microscopy, Phase-Contrast, Osmium Tetroxide, Synchrotrons, Tomography, X-Ray Computed, X-Ray Microtomography, Radiology, Nuclear Medicine and Medical Imaging, Radiologi och bildbehandling, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1111.jmi.13005 |
| URL: | Journal of Microscopy, 0022-2720, 2021, 283:1, s. 29-40 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:kth-309236 0000-0002-6716-1795 0000-0003-2723-6622 doi:10.1111/jmi.13005 PMID 33822371 ISI:000648344300001 Scopus 2-s2.0-85105346424 1312824745 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1312824745 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1111.jmi.13005 |
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