Silibinin Triggers the Mitochondrial Pathway of Apoptosis in Human Oral Squamous Carcinoma Cells
| العنوان: | Silibinin Triggers the Mitochondrial Pathway of Apoptosis in Human Oral Squamous Carcinoma Cells |
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| المؤلفون: | Ezhilarasan Devaraj, Radhika Murali Iyangar |
| المصدر: | Asian Pacific Journal of Cancer Prevention : APJCP |
| بيانات النشر: | West Asia Organization for Cancer Prevention, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cytotoxicity, Cytochrome c, Silibinin, Apoptosis, Antioxidants, Silybum marianum, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Tumor Cells, Cultured, Cytotoxic T cell, Humans, MTT assay, biology, Squamous Cell Carcinoma of Head and Neck, General Medicine, biology.organism_classification, Squamous carcinoma, Mitochondria, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, Caspases, Cancer research, biology.protein, Mouth Neoplasms, Research Article, Silymarin |
| الوصف: | Background Silibinin, a natural polyphenolic flavonoid present in seed extracts of milk thistle (Silybum marianum). It has been shown to interact with various cancer-related cell signalling pathways in preclinical models, demonstrating promising anticancer effects in vitro and in vivo. Materials and methods The cytotoxic effect of silibinin was evaluated in human oral squamous carcinoma (SCC-25) cells by MTT assay. The apoptosis-related morphological changes were investigated by AO/EB dual staining. The cytochrome c, caspases-3, and -9, B-cell lymphoma-2 (Bcl-2), and B-cell associated X protein (Bax) gene expressions were analysed by PCR. Results We have shown that silibinin treatment for 24 h in SCC-25 cells induced cytotoxicity in a concentration-dependent manner. The cytotoxic potential was due to the induction of apoptosis via the release of mitochondrial cytochrome c into the cytosol and subsequent activation of caspases-3 and -9. Dual staining assay was further confirmed the induction of early apoptosis upon silibinin treatment. Conclusion The results from this study show that silibinin can be considered as a promising drug candidate for the treatment of oral squamous cell carcinoma. |
| اللغة: | English |
| تدمد: | 2476-762X 1513-7368 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::543371ef53f07bfded574b4c2cc2b96eTest http://europepmc.org/articles/PMC7573425Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....543371ef53f07bfded574b4c2cc2b96e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2476762X 15137368 |
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