A technology platform for digital nucleic acid diagnostics at the point of care
| العنوان: | A technology platform for digital nucleic acid diagnostics at the point of care |
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| المؤلفون: | Ana Belén Rodríguez-Martínez, Simon Wadle, Lisa Becherer, Jia Li, Mara Specht, Martin Trotter, Friedrich Schuler, Martin Schulz, Roland Zengerle, Nadine Borst, Felix von Stetten, Nils Paust |
| المصدر: | LaboratoriumsMedizin. 41:245-249 |
| بيانات النشر: | Walter de Gruyter GmbH, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 010401 analytical chemistry, Biochemistry (medical), Clinical Biochemistry, 02 engineering and technology, Computational biology, Lab-on-a-chip, 021001 nanoscience & nanotechnology, Bioinformatics, 01 natural sciences, 0104 chemical sciences, law.invention, Medical Laboratory Technology, law, Nucleic acid, 0210 nano-technology, Point of care |
| الوصف: | The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, including PCR, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), have already been demonstrated. Our novel approach of integrated sample-to-answer analysis is introduced, and examples for the detection of HIV and single cell analysis of antibiotic resistant bacteria are presented. Next to these LabDisk based systems, a microfluidic cartridge termed DropChip allows for digital amplification using only commercially available laboratory devices. |
| تدمد: | 1439-0477 0342-3026 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::6bb7500ea43dd754e7e14dc4f7cad3ebTest https://doi.org/10.1515/labmed-2017-0098Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........6bb7500ea43dd754e7e14dc4f7cad3eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390477 03423026 |
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