Alleviation of enterotoxigenicEscherichia colichallenge by recombinantLactobacillus plantarumexpressing a FaeG- and DC-targeting peptide fusion protein
| العنوان: | Alleviation of enterotoxigenicEscherichia colichallenge by recombinantLactobacillus plantarumexpressing a FaeG- and DC-targeting peptide fusion protein |
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| المؤلفون: | B. Shan, Chun-Wei Shi, Chun-Feng Wang, Wei Gu, Jingtao Hu, Liping Ye, Gui-Lian Yang, P. Tong, Yanlong Jiang, W. Yang, C. Li |
| المصدر: | Beneficial Microbes. 8:379-391 |
| بيانات النشر: | Wageningen Academic Publishers, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Immunoglobulin A, Recombinant Fusion Proteins, Fimbria, medicine.disease_cause, Microbiology, Bacterial Adhesion, Immunoglobulin G, Mice, 03 medical and health sciences, Immune system, Antigen, Enterotoxigenic Escherichia coli, medicine, Animals, Adhesins, Escherichia coli, B-Lymphocytes, Mice, Inbred BALB C, biology, Probiotics, Dendritic Cells, biology.organism_classification, Fusion protein, 030104 developmental biology, biology.protein, bacteria, Female, Lactobacillus plantarum |
| الوصف: | FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19+B cells and CD11c+DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate. |
| تدمد: | 1876-2891 1876-2883 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14e0137957fce0890b28c525fc4ee94dTest https://doi.org/10.3920/bm2016.0116Test |
| رقم الانضمام: | edsair.doi.dedup.....14e0137957fce0890b28c525fc4ee94d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18762891 18762883 |
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