دورية أكاديمية
Effect of etanercept and lithium chloride on preventing secondary tissue damage in rats with experimental diffuse severe brain injury
| العنوان: | Effect of etanercept and lithium chloride on preventing secondary tissue damage in rats with experimental diffuse severe brain injury |
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| المؤلفون: | Ekici, Mehmet Ali, Çıkrıklar, Halil İbrahim, Uysal, Onur, Özbek, Zühtü, Turgut, Didem Coşan, Baydemir, Canan, Kazancı, Burak |
| المساهمون: | Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Sağlığı ve Hastalıkları Anabilim Dalı., Hafızoğlu, Demet, 36711582000 |
| بيانات النشر: | Verduci Publisher |
| سنة النشر: | 2014 |
| المجموعة: | Açık Erişim@BUU (Bursa Uludağ Üniversitesi) |
| مصطلحات موضوعية: | Traumatic brain injury, Etanercept, Lithium chloride, TNF-alpha, Necrosis-factor-alpha, Spinal-cord-injury, Fibrillary acidic protein, Focal serebral-ischemia, Tau-protein, Rheumatoid-arthritis, Neuronal damage, Expression, Model, Pharmacology & pharmacy, Animals, Apoptosis, Astrocytes, Brain, Brain injuries, Disease models, animal, Drug therapy, Combination, Glial fibrillary acidic protein, Immunoglobulin G, Male, Neurons, Neuroprotective agents, Rats, sprague-dawley |
| الوصف: | OBJECTIVE: Studies in animals have provided key evidence that antagonizing TNF-alpha is a viable therapeutic strategy for diffuse severe brain injury. This study is planned to prevent post-traumatic secondary tissue damages in rat diffuse severe brain injury model, which is induced by alone or combined administration of Etanercept and lithium chloride (LiCl). MATERIALS AND METHODS: Male SpragueDawley rats were used in the current study. Rats were divided into 5 groups. Trauma was not induced and treatment was not applied to rats of Sham group. For rats of Trauma+Saline group, saline 0.9% was administered via intraperitoneal (i.p.) route at dose of 1 mg/100 g body weight 1 hour after trauma. For rats of Trauma+Etanercept group, Etanercept was administered via i.p. route at dose of 5 mg/kg body weight 1 hour after trauma. For rats of Trauma+LiCl group, LiCl was administered via i.p. route at dose of 50 mg/kg body weight 1 hour after trauma. For rats of Etanercept+LiCl group, Etanercept and LiCl were administered via i.p. route at dose of 5 mg/kg body weight and 50 mg/kg body weight, respectively, 1 hour after trauma. Serum glial fibrillary acidic protein (GFAP) and Tau levels were analyzed with ELISA. For analyses H&E, TUNEL, GFAP and TNF-alpha staining methods were used. RESULTS: We demonstrate that Etanercept treatment reduced the TBI-induced brain tissues alteration, reduced the expression of TNF-alpha and improve edema and axonal swelling. We observed a significant decrease in TNF-alpha and GFAP positivity after LiCl was administered. CONCLUSIONS: The findings obtained in this study suggest that the combination therapy with Etanercept and LiCl decreased neuronal degeneration and alleviated secondary tissue damage in post-traumatic period. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-331436-6 0-00-331436-7 |
| تدمد: | 1128-3602 |
| العلاقة: | Makale - Uluslararası Hakemli Dergi; European Review for Medical and Pharmacological Sciences; Yurt içi; Sanayi; Ekici, M. A. vd. (2014). "Effect of etanercept and lithium chloride on preventing secondary tissue damage in rats with experimental diffuse severe brain injury". European Review for Medical and Pharmacological Sciences, 18(1), 10-27.; https://www.europeanreview.org/article/6443Test; http://hdl.handle.net/11452/28781Test; 000331436700002; 2-s2.0-84896953262; 10; 27; 18 |
| الإتاحة: | http://hdl.handle.net/11452/28781Test https://www.europeanreview.org/article/6443Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.86E33544 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780003314366 0003314367 |
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| تدمد: | 11283602 |