دورية أكاديمية
Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium
| العنوان: | Effect of rhBMP-2 applied with a 3D-printed titanium implant on new bone formation in rabbit calvarium |
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| المؤلفون: | Jaeyoung RYU, Hyo-Sun KANG, Byung-Hun KANG, Seunggon JUNG, Min-Suk KOOK, Hee-Kyun OH, Ji-Yeon JUNG, Hong-Ju PARK |
| المصدر: | Journal of Applied Oral Science, Vol 29 (2021) |
| بيانات النشر: | University of São Paulo, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Dentistry |
| مصطلحات موضوعية: | Three-dimensional printing, Bone morphogenetic protein, Osteogenesis, Dentistry, RK1-715 |
| الوصف: | Abstract Objective This study sought to compare the biocompatibility of a three-dimensional (3D)-printed titanium implant with a conventional machined titanium product, as well as the effect of such implant applied with recombinant human Bone Morphogenetic Protein Type 2 (rhBMP-2) for guided bone regeneration. Methodology Disk-shaped titanium specimens fabricated either by the conventional machining technique or by the 3D-printing technique were compared by MC3T3-E1 cells cytotoxicity assay. New bone formation was evaluated using a rapid prototype titanium cap applied to the calvaria of 10 rabbits, which were divided into two groups: one including an atelopeptide collagen plug on one side of the cap (group I) and the other including a plug with rhBMP-2 on the other side (group II). At six and 12 weeks after euthanasia, rabbits calvaria underwent morphometric analysis through radiological and histological examination. Results Through the cytotoxicity assay, we identified a significantly higher number of MC3T3-E1 cells in the 3D-printed specimen when compared to the machined specimen after 48 hours of culture. Moreover, morphometric analysis indicated significantly greater bone formation at week 12 on the side where rhBMP-2 was applied when evaluating the upper portion immediately below the cap. Conclusion The results suggest that 3D-printed titanium implant applied with rhBMP-2 enables new bone formation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-7765 1678-7757 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100440&tlng=enTest; https://doaj.org/toc/1678-7765Test |
| DOI: | 10.1590/1678-7757-2020-1092 |
| الوصول الحر: | https://doaj.org/article/1318fe7c8e614cfeade1a736916a7b43Test |
| رقم الانضمام: | edsdoj.1318fe7c8e614cfeade1a736916a7b43 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16787765 16787757 |
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| DOI: | 10.1590/1678-7757-2020-1092 |