التفاصيل البيبلوغرافية
العنوان: |
Pyrimidine Enzyme Specific Activity at Four Different Phases of Growth in Minimal and Rich Media, and Concomitant Virulence Factors Evaluation in Pseudomonas aeruginosa |
المؤلفون: |
Azad, Kamran Nikkhah |
المساهمون: |
O'Donovan, Gerard A., Benjamin, Robert C., Knesek, John, Schafer, Rollie, Shanley, Mark S. |
بيانات النشر: |
University of North Texas |
سنة النشر: |
2005 |
المجموعة: |
University of North Texas: UNT Digital Library |
مصطلحات موضوعية: |
Pyrimidine nucleotides -- Metabolism, Pseudomonas aeruginosa, pyrimidine pathway, four phases of growth, virulence factor production, minimal and rich media, enzyme specific activity |
الوصف: |
Pseudomonas aeruginosa is a Gram-negative rod, aerobic, non-fermenting, oxidase positive, pigment producing, and nutritionally versatile bacterium. Infections by P. aeruginosa are the most important cause of morbidity and mortality in immunocompromised patients, given virulence factor production that suppresses antibiotic therapy and promotes persistent infection. This research is the first comprehensive report of the pyrimidine biosynthetic pathway for all phases of growth in minimal and rich media coupled with the evaluation of virulence factor production of P. aeruginosa in comparison to four other bacterial species (Pseudomonas putida, Pseudomonas fluorescens, Burkholderia cepacia, and Escherichia coli wild-type strains). Cellular growth and passing genetic information to the next generation depend on the synthesis of purines and pyrimidines, the precursors of DNA and RNA. The pyrimidine biosynthetic pathway is essential and found in most organisms, with the exception of a few parasites that depend upon the pyrimidine salvage pathway for growth. Both the pyrimidine biosynthetic and salvage enzymes are targets for chemotherapeutic agents. In our laboratory, research on pyrimidine auxotrophic mutants showed the role of the pyrimidine biosynthetic pathway and its intermediates on P. aeruginosa metabolism and impaired virulence factors production. The present research shows that pyrimidine enzymes are active in all phases of growth, including the production of two forms of ATCase in the late log phase in P. aeruginosa. This finding may be explained by the displacement of the inactive PyrC' by the active PyrC or PyrC2 to form a new and larger pyrBC encoded ATCase. Pseudomonas aeruginosa wild-type appears to produce by far the most virulence factors, haemolysin, iron chelation, rhamnolipid, adherence, and three types of motility (swimming, swarming, and twitching) investigated in this study, when compared to the other four wild-type strains. Growth analysis was carried out as typically done in minimal medium but ... |
نوع الوثيقة: |
text |
وصف الملف: |
text |
اللغة: |
English |
العلاقة: |
oclc: 69659192; http://digital.library.unt.edu/ark:/67531/metadc4918Test/; ark: ark:/67531/metadc4918 |
الإتاحة: |
http://digital.library.unt.edu/ark:/67531/metadc4918Test/ |
حقوق: |
unt ; copyright ; Azad, Kamran Nikkhah ; Copyright is held by the author, unless otherwise noted. All rights reserved. |
رقم الانضمام: |
edsbas.60E6F296 |
قاعدة البيانات: |
BASE |