رسالة جامعية
Lipogenic Proteins in Plants: Functional Homologues and Applications
| العنوان: | Lipogenic Proteins in Plants: Functional Homologues and Applications |
|---|---|
| المؤلفون: | Cai, Yingqi |
| مرشدي الرسالة: | Chapman, Kent D., Ayre, Brian G., Dickstein, Rebecca, Shah, Jyoti, Shulaev, Vladimir |
| بيانات النشر: | University of North Texas, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | University of North Texas |
| Original Material: | local-cont-no: submission_1366 ark: ark:/67531/metadc1404563 |
| مصطلحات موضوعية: | lipid droplet, endoplasmic reticulum, SEIPIN, fat storage-inducing transmembrane protein 2, triacylglycerol, terpene, Lipids, Metabolism., Endoplasmic reticulum., Terpenes., Membrane proteins. |
| الوصف: | Although cytoplasmic lipid droplets (LDs) are the major reserves for energy-dense neutral lipids in plants, the cellular mechanisms for packaging neutral lipids into LDs remain poorly understood. To gain insights into the cellular processes of neutral lipid accumulation and compartmentalization, a necessary step forward would be to characterize functional roles of lipogenic proteins that participate in the compartmentalization of neutral lipids in plant cells. In this study, the lipogenic proteins, Arabidopsis thaliana SEIPIN homologues and mouse (Mus Musculus) fat storage-inducing transmembrane protein 2 (FIT2), were characterized for their functional roles in the biogenesis of cytoplasmic LDs in various plant tissues. Both Arabidopsis SEIPINs and mouse FIT2 supported the accumulation of neutral lipids and cytoplasmic LDs in plants. The three Arabidopsis SEIPIN isoforms play distinct roles in compartmentalizing neutral lipids by enhancing the numbers and sizes of LDs in various plant tissues and developmental stages. Further, the potential applications of Arabidopsis SEIPINs and mouse FIT2 in engineering neutral lipids and terpenes in plant vegetative tissues were evaluated by co-expressing these and other lipogenic proteins in Nicotiana benthamiana leaves. Arabidopsis SEIPINs and mouse FIT2 represent effective tools that may complement ongoing strategies to enhance the accumulation of desired neutral lipids and terpenes in plant vegetative tissues. Collectively, our findings in this study expand our knowledge of the broader cellular mechanisms of LD biogenesis that are partially conserved in eukaryotes and distinct in plants and suggest novel targets that can be introduced into plants to collaborate with other factors in lipid metabolism and elevate oil content in plant tissues. |
| Original Identifier: | info:ark/67531/metadc1404563 |
| نوع الوثيقة: | Thesis or Dissertation |
| وصف الملف: | viii, 163 pages; Text |
| اللغة: | English |
| الإتاحة: | https://digital.library.unt.edu/ark:/67531/metadc1404563Test/ |
| حقوق: | Public Cai, Yingqi Copyright is held by the author, unless otherwise noted. All rights Reserved. |
| رقم الانضمام: | edsndl.unt.edu.info.ark.67531.metadc1404563 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |