The intensive use of antibiotics in aquaculture results in the proliferation of antibiotic. In this study,antibiotic resistantbacteria from six different aquaculture sources (pond of Fisheries Research Institute (FRI), and rivers of Kukup, Pulai, Pendas Laut, Sungai Melayu and Kong Kong) were isolated. These isolates were tested for antibiotic re-sistance against sevenantibiotics via the disc diffusion method. Finally, phenotypic and genotypic identification via 16S rRNA sequencing and phylogenetic analysis were carried out. The results show that 58 out of 118 bacterial isolates are resistant to multiple antibiotics. The highest isolate resistance was observed towards rifampicin (89.66%), followed by ampicillin (79.31%) and sulfafurazole (67.24%). The isolates with multiple antibiotic resistant (MAR) index valueswith more than 20% were sub-jected to 16S rRNA gene sequencing. The majority of the bacterial strains exhibit multiple antibiotic resistance, indicating that they were isolated from highly contami-nated sources based on the tested water qualities profiles, which showed the high level of turbidity and total dissolved solid (TDS) in mostsampling sites with the high num-ber of MAR bacteria obtained.
