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1دورية أكاديمية
المؤلفون: Pires, Ananda da Rocha, Gerardi, Daniel, Tisotti, Tainor de Mesquita, Serpa, Priscila Beatriz da Silva, Natalini, Cláudio Corrêa
المصدر: Ciência Rural. January 2021 51(10)
مصطلحات موضوعية: ABCB1, canine, P-glycoprotein
الوصف: The P-glycoprotein (P-gp) is a transmembrane protein encoded by the MDR1 gene that functions as a biological barrier by extruding toxins and xenobiotics out of cells. The MDR1 gene can carry a mutation called nt230(del4), which is a deletion of four base pairs resulting in the formation of a non-functional protein that may predispose to severe toxicosis, as observed in dogs with sensitivity to ivermectin. Several breeds have been described as carriers of the mutation, including German Shepherds (GS). However, the presence of the mutant allele in this breed has not been described in Brazil. This study aimed to determine the genotypic and allelic frequency of the nt230(del4) mutation in the MDR1 gene in GS from Southern Brazil. Blood samples were collected from 79 GS in the state of Rio Grande do Sul and genotype for the MDR1 gene was performed. Seventy-eight (98.7%) dogs were dominant homozygous genotype (wild) and one (1.3%) was heterozygous. This study showed that there is a low frequency (0.6%) of the mutant allele while the frequency of the wild allele is high (99.4%) in this specific population. This is the first report of the presence of the nt230(del4) mutation in the MDR1 gene in GS in Brazil. This information is important for breeders to prevent dissemination of the mutant allele in the national breeding population and international exchange of animals for breeding; for owners and veterinarians to be aware when dispensing and administering medications for GS dogs in Brazil.
وصف الملف: text/html
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2دورية أكاديمية
المؤلفون: Ananda da Rocha Pires, Daniel Gerardi, Tainor de Mesquita Tisotti, Priscila Beatriz da Silva Serpa, Cláudio Corrêa Natalini
المصدر: Ciência Rural, Vol 51, Iss 10 (2021)
مصطلحات موضوعية: ABCB1, canine, P-glycoprotein, Agriculture, Agriculture (General), S1-972
الوصف: ABSTRACT: The P-glycoprotein (P-gp) is a transmembrane protein encoded by the MDR1 gene that functions as a biological barrier by extruding toxins and xenobiotics out of cells. The MDR1 gene can carry a mutation called nt230(del4), which is a deletion of four base pairs resulting in the formation of a non-functional protein that may predispose to severe toxicosis, as observed in dogs with sensitivity to ivermectin. Several breeds have been described as carriers of the mutation, including German Shepherds (GS). However, the presence of the mutant allele in this breed has not been described in Brazil. This study aimed to determine the genotypic and allelic frequency of the nt230(del4) mutation in the MDR1 gene in GS from Southern Brazil. Blood samples were collected from 79 GS in the state of Rio Grande do Sul and genotype for the MDR1 gene was performed. Seventy-eight (98.7%) dogs were dominant homozygous genotype (wild) and one (1.3%) was heterozygous. This study showed that there is a low frequency (0.6%) of the mutant allele while the frequency of the wild allele is high (99.4%) in this specific population. This is the first report of the presence of the nt230(del4) mutation in the MDR1 gene in GS in Brazil. This information is important for breeders to prevent dissemination of the mutant allele in the national breeding population and international exchange of animals for breeding; for owners and veterinarians to be aware when dispensing and administering medications for GS dogs in Brazil.
وصف الملف: electronic resource
العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000602&tlng=enTest; https://doaj.org/toc/1678-4596Test
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3دورية أكاديمية
المصدر: Ciência Rural. February 2006 36(1)
الوصف: Opioid absorption in the intestinal tract as well as its effects in the central nervous system is modulated by the P-glycoprotein (P-gp) encoded in the Multi-drug Resistance gene (MDR1) also named ATP-binding cassete, subfamily B, member 1 (ABCB1). This MDR1 gene acts as a selective pump. The expression of this protein in humans and rodents inhibits cellular uptake of substrate opioids. The presence of the intestinal iso-enzyme CYP3A4 associated with MDR1 gene decreases the opioid analgesic activity due to an increase in intestinal metabolism, with a predicted intestinal first pass extraction around 20% which significantly influences the oral availability of opioids. In the central nervous system, P-gp expression decreases opioid neuronal uptake diminishing the analgesic effects. It is unknown if horses have the MDR1 gene and P-gp and what are the effects on opioid absorption, metabolism, and analgesia. Identifying the MDR1 gene and P-gp status in horses is of great importance in order to better understand opioid pharmacologic effects in horses.
وصف الملف: text/html
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4دورية أكاديمية
المؤلفون: Natalini, Cláudio Corrêa, Linardi, Renata Lehn
المصدر: Ciência Rural. February 2006 36(1)
الوصف: P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on drugs pharmacologic effects in horses are granted.
وصف الملف: text/html
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5
المؤلفون: Tainor de Mesquita Tisotti, Ananda da Rocha Pires, Priscila B S Serpa, Daniel Guimarães Gerardi, Claudio Correa Natalini
المصدر: Ciência Rural, Volume: 51, Issue: 10, Article number: e20200690, Published: 14 JUN 2021
Ciência Rural, Vol 51, Iss 10 (2021)
Ciência Rural v.51 n.10 2021
Ciência Rural
Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGSمصطلحات موضوعية: 0106 biological sciences, 040301 veterinary sciences, Homozygous genotype, Cães, Agriculture (General), Population, canine, Biology, P-glycoprotein, medicine.disease_cause, 01 natural sciences, S1-972, 0403 veterinary science, Glicoproteína-P, 010608 biotechnology, Genotype, medicine, Mutação genética, education, Allele frequency, Administering medications, education.field_of_study, Mutation, General Veterinary, Pastor Alemão, Brasil, Região Sul, Agriculture, ABCB1, 04 agricultural and veterinary sciences, Mdr1 gene, International exchange, Molecular biology, Glicoproteina P, canino, Animal Science and Zoology, Agronomy and Crop Science
الوصف: The P-glycoprotein (P-gp) is a transmembrane protein encoded by the MDR1 gene that functions as a biological barrier by extruding toxins and xenobiotics out of cells. The MDR1 gene can carry a mutation called nt230(del4), which is a deletion of four base pairs resulting in the formation of a non-functional protein that may predispose to severe toxicosis, as observed in dogs with sensitivity to ivermectin. Several breeds have been described as carriers of the mutation, including German Shepherds (GS). However, the presence of the mutant allele in this breed has not been described in Brazil. This study aimed to determine the genotypic and allelic frequency of the nt230(del4) mutation in the MDR1 gene in GS from Southern Brazil. Blood samples were collected from 79 GS in the state of Rio Grande do Sul and genotype for the MDR1 gene was performed. Seventy-eight (98.7%) dogs were dominant homozygous genotype (wild) and one (1.3%) was heterozygous. This study showed that there is a low frequency (0.6%) of the mutant allele while the frequency of the wild allele is high (99.4%) in this specific population. This is the first report of the presence of the nt230(del4) mutation in the MDR1 gene in GS in Brazil. This information is important for breeders to prevent dissemination of the mutant allele in the national breeding population and international exchange of animals for breeding; for owners and veterinarians to be aware when dispensing and administering medications for GS dogs in Brazil. RESUMO: A Glicoproteína-P é uma proteína transmembrana codificada pelo gene MDR1 que atua como uma barreira fisiológica através da extrusão de toxinas e xenobióticos para fora das células. O gene MDR1 pode carregar uma mutação chamada nt230(del4) que é uma deleção de quatro pares de bases, resultando na formação de uma proteína não-funcional que pode predispor à toxicoses graves, como as observadas em cães sensíveis à ivermectina. Diversas raças de cães foram descritas como portadoras da mutação nt230(del4), incluindo Pastores Alemães (PA). Entretanto, a presença do alelo mutante nessa raça não foi descrita em cães no Brasil. O objetivo desse estudo foi determinar a frequência genotípica e alélica da mutação nt230(del4) em PA no sul do Brasil. Amostras de sangue foram coletadas de 79 PA no estado do Rio Grande do Sul e o genótipo dos cães para o gene MDR1 realizado. Setenta e oito (98.7%) cães foram homozigotos dominantes (selvagem) e um (1.3%) tinha genótipo heterozigoto. A frequência do alelo mutante foi baixa (0.6%), enquanto a frequência do alelo selvagem foi alta (99.4%) nesta população. Este é o primeiro relato da presença desta mutação nt230(del4) no gene MDR1 em PA no Brasil. Esta informação é importante para criadores a fim de prevenir a disseminação do alelo mutante na população de criadores da raça no Brasil e programas internacionais de troca de animais para criação, para tutores e veterinários estarem conscientes quando prescreverem e administrarem medicações para cães PA no Brasil.
وصف الملف: text/html; application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdec8ee4dae1b65475952da0b876d371Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021001000602&lng=en&tlng=enTest -
6دورية أكاديمية
المصدر: Ciência Rural, Vol 36, Iss 1, Pp 330-335 (2006)
مصطلحات موضوعية: molecular biology, opioids, equine, gene, MDR1, ABCB1, analgesia, Agriculture, Agriculture (General), S1-972
الوصف: Opioid absorption in the intestinal tract as well as its effects in the central nervous system is modulated by the P-glycoprotein (P-gp) encoded in the Multi-drug Resistance gene (MDR1) also named ATP-binding cassete, subfamily B, member 1 (ABCB1). This MDR1 gene acts as a selective pump. The expression of this protein in humans and rodents inhibits cellular uptake of substrate opioids. The presence of the intestinal iso-enzyme CYP3A4 associated with MDR1 gene decreases the opioid analgesic activity due to an increase in intestinal metabolism, with a predicted intestinal first pass extraction around 20% which significantly influences the oral availability of opioids. In the central nervous system, P-gp expression decreases opioid neuronal uptake diminishing the analgesic effects. It is unknown if horses have the MDR1 gene and P-gp and what are the effects on opioid absorption, metabolism, and analgesia. Identifying the MDR1 gene and P-gp status in horses is of great importance in order to better understand opioid pharmacologic effects in horses.
وصف الملف: electronic resource
العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100055&tlng=enTest; http://www.scielo.br/pdf/cr/v36n1/a55v36n1.pdfTest; https://doaj.org/toc/1678-4596Test
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7دورية أكاديمية
المؤلفون: Cláudio Corrêa Natalini, Renata Lehn Linardi
المصدر: Ciência Rural, Vol 36, Iss 1, Pp 298-300 (2006)
مصطلحات موضوعية: molecular biology, opioids, equine, gene, MDR1, ABCB1, Agriculture, Agriculture (General), S1-972
الوصف: P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on drugs pharmacologic effects in horses are granted.
وصف الملف: electronic resource
العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048&tlng=enTest; http://www.scielo.br/pdf/cr/v36n1/a48v36n1.pdfTest; https://doaj.org/toc/1678-4596Test
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8
المصدر: Ciência Rural, Vol 36, Iss 1, Pp 330-335 (2006)
Ciência Rural v.36 n.1 2006
Ciência Rural
Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
Ciência Rural, Volume: 36, Issue: 1, Pages: 330-335, Published: FEB 2006مصطلحات موضوعية: Agriculture (General), Central nervous system, Analgesic, MDR1, Drug resistance, Pharmacology, S1-972, lcsh:Agriculture, opióides, medicine, molecular biology, Intestinal Metabolism, lcsh:Agriculture (General), gene, Gene, equine, General Veterinary, CYP3A4, biologia molecular, business.industry, lcsh:S, opioids, ABCB1, analgesia, Agriculture, Metabolism, lcsh:S1-972, medicine.anatomical_structure, Opioid, Animal Science and Zoology, business, Agronomy and Crop Science, eqüino, medicine.drug
الوصف: Opioid absorption in the intestinal tract as well as its effects in the central nervous system is modulated by the P-glycoprotein (P-gp) encoded in the Multi-drug Resistance gene (MDR1) also named ATP-binding cassete, subfamily B, member 1 (ABCB1). This MDR1 gene acts as a selective pump. The expression of this protein in humans and rodents inhibits cellular uptake of substrate opioids. The presence of the intestinal iso-enzyme CYP3A4 associated with MDR1 gene decreases the opioid analgesic activity due to an increase in intestinal metabolism, with a predicted intestinal first pass extraction around 20% which significantly influences the oral availability of opioids. In the central nervous system, P-gp expression decreases opioid neuronal uptake diminishing the analgesic effects. It is unknown if horses have the MDR1 gene and P-gp and what are the effects on opioid absorption, metabolism, and analgesia. Identifying the MDR1 gene and P-gp status in horses is of great importance in order to better understand opioid pharmacologic effects in horses. A absorção de opióides no trato intestinal, assim como seus efeitos no sistema nervoso central, são modulados pela P-glicoproteína (P-gp), uma proteína de membrana celular codificada pelo gene MDR1, também chamado ATP-binding cassete, subfamília B, membro 1 (ABCB1) e que atua como bomba seletiva. A expressão desta proteína em roedores e seres humanos inibe a absorção celular de opióides e sua presença no intestino associada à isoenzima CYP3A4 reduz a atividade analgésica dos opióides por ativação do metabolismo intestinal do fármaco. A redução na extração intestinal de fármacos opióides susceptíveis a esta proteína chega a 20%, o que reduz significativamente a biodisponibilidade de opióides administrados por via oral. No sistema nervoso central, a P-gp diminui a captação neuronal dos opióides e seus efeitos analgésicos. Ainda é desconhecido se o gene MDR1 e a P-gp estão presentes no trato intestinal e no sistema nervoso central em cavalos e quais os seus efeitos na absorção, metabolismo e efeito analgésico nesta espécie. Fica evidente a importância da determinação da presença ou não deste gene e sua expressão protéica no cavalo, para um melhor entendimento da farmacologia dos opióides nesta espécie.
وصف الملف: text/html
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da0b88d7560860c0c773bcb49d80af55Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100055&tlng=enTest -
9
المؤلفون: Renata Lehn Linardi, Cláudio Corrêa Natalini
المصدر: Ciência Rural, Vol 36, Iss 1, Pp 298-300 (2006)
Ciência Rural v.36 n.1 2006
Ciência Rural
Universidade Federal de Santa Maria (UFSM)
instacron:UFSMمصطلحات موضوعية: Agriculture (General), Central nervous system, Ileum, MDR1, Drug resistance, Pharmacology, Biology, physiological processes, S1-972, lcsh:Agriculture, Pharmacokinetics, medicine, polycyclic compounds, molecular biology, lcsh:Agriculture (General), gene, Gene, neoplasms, equine, Gastrointestinal tract, General Veterinary, lcsh:S, opioids, Transporter, Agriculture, ABCB1, lcsh:S1-972, Bioavailability, medicine.anatomical_structure, Animal Science and Zoology, Agronomy and Crop Science
الوصف: P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on drugs pharmacologic effects in horses are granted.
وصف الملف: text/html
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a4880fc614b0a0da75d346db82055d3Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048Test
