Protein PEGylation for the design of biobetters: from reaction to purification processes
| العنوان: | Protein PEGylation for the design of biobetters: from reaction to purification processes |
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| المؤلفون: | João H. P. M. Santos, Carlota de Oliveira Rangel-Yagui, Beatriz Panichi Amaro, Giovanna Pastore Meneguetti, Karin Torres-Obreque |
| المصدر: | Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. Especial (2018); e01009 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. Especial (2018); e01009 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. Especial (2018); e01009 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 54, Iss spe (2018) |
| بيانات النشر: | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Polyethylene glycol, lcsh:RS1-441, 02 engineering and technology, lcsh:Pharmacy and materia medica, 03 medical and health sciences, chemistry.chemical_compound, Site-specific PEGylation, Protein purification, Biobetters, Bioprocess, Biological drugs, Downstream processing, Sitespecific PEGylation, Chemistry, Proteolytic enzymes, PEGylation, 021001 nanoscience & nanotechnology, 030104 developmental biology, Protein drug, Biochemical engineering, 0210 nano-technology, Conjugate |
| الوصف: | The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of structure and activity of the resulting species. These steps are not trivial tasks, reason why bioprocessing operations based on PEGylated proteins relies on the use of analytical tools according to the specific pharmaceutical conjugate that is being developed. Therefore, the appropriate selection of the technical and analytical methods may ensure success in implementing a feasible industrial process. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2175-9790 1984-8250 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cce43152ab4d2e639308793d7726bf3Test https://www.revistas.usp.br/bjps/article/view/153908Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2cce43152ab4d2e639308793d7726bf3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21759790 19848250 |
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