رسالة جامعية
Contribución de los sistemas toxina-antitoxina de Salmonella enterica serovar Typhimurium en la adaptación a la vida intracelular
| العنوان: | Contribución de los sistemas toxina-antitoxina de Salmonella enterica serovar Typhimurium en la adaptación a la vida intracelular |
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| المؤلفون: | Lobato-Márquez, Damián |
| المساهمون: | Díaz-Orejas, Ramón, García del Portillo, Francisco, Ministerio de Educación, Cultura y Deporte (España) |
| بيانات النشر: | CSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB) Universidad Autónoma de Madrid |
| سنة النشر: | 2015 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Salmonella, Infección, Sistemas Toxina-Antitoxina |
| الوصف: | 198p.-28 fig.-5 tab.-3 anexos ; [EN] Toxin‐antitoxin modules (hereafter TA) are operons composed of two small genes encoding an unstable antitoxin and a stable toxin. TA loci abound in microbial genomes and are found in free--‐ living, symbiotic and obligate intracellular bacteria. Toxins exhibit activities ranging from RNAses to DNA gyrase inhibitors, and mainly trigger bacteriostatic effects. TA modules are implicated in processes such as survival in response to nutrient starvation, response to oxidative damage, biofilm formation or tolerance to antibiotics. In addition, recent studies have focused in understanding whether TA modules contribute to pathogen survival during infection. Thus, uropathogenic Escherichia coli, Haemophilus influenzae and Salmonella enterica serovar Typhimurium (S. Typhimurium) use TA modules to colonize and survive in animal organs. S. enterica is an intracellular bacterial pathogen that can cause persistent infections in humans and livestock. The serovar Typhimurium has been extensively studied in murine models in which the pathogen causes either acute or chronic infections. In the animal, S. Typhimurium targets preferentially macrophages, but can also infect other cell types like fibroblasts, where this pathogen attenuates its intracellular growth rate. The aim of this work was to determine the possible role of S. Typhimurium TA modules during the infection and survival within eukaryotic cells. Twenty--‐seven putative TA modules were identified using different bioinformatic approaches. Functional assays, based on the growth inhibition caused by the toxin and the neutralization of this effect by the antitoxin, showed that 18 TA modules were bona fide systems. It was observed that some TA modules are expressed by intracellular bacteria during fibroblast but not epithelial cell infection. Moreover, only five out of ten TA systems for which defective mutants were generated showed a clear reduction in the survival ability of intracellular S. Typhimurium in the fibroblast infection model, ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | Spanish; Castilian |
| العلاقة: | Publisher's version; Toxins 2015, 7, 478-492 https://digital.csic.es/handle/10261/116260Test; Scientific Reports 2015, 5 (9374) https://digital.csic.es/handle/10261/113343Test; Sí; http://hdl.handle.net/10261/137216Test; http://dx.doi.org/10.13039/501100003176Test |
| DOI: | 10.13039/501100003176 |
| الإتاحة: | https://doi.org/10.13039/501100003176Test http://hdl.handle.net/10261/137216Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.31AF6D08 |
| قاعدة البيانات: | BASE |
| DOI: | 10.13039/501100003176 |
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