دورية أكاديمية
The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing
| العنوان: | The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing |
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| المؤلفون: | Makeyev, EV, Maniatis, T, Zhang, J, Carrasco, MA |
| بيانات النشر: | //www.elsevier.com/locate/molcel United States |
| سنة النشر: | 2007 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Cells, Cultured, Animals, Cell Differentiation, Neurons - Cytology - Metabolism, Neuroblastoma - Genetics - Metabolism - Pathology, Nerve Tissue Proteins - Genetics - Metabolism, Mitosis - Physiology, Micrornas - Genetics - Metabolism, Mice, Humans, Heterogeneous-Nuclear Ribonucleoproteins - Genetics - Metabolism, Gene Expression Regulation, Developmental, Exons, Alternative Splicing - Genetics, Transfection, Rna, Messenger - Chemistry - Metabolism, Rna Processing, Post-Transcriptional, Brain - Physiology, Rna Precursors - Genetics - Metabolism, Polypyrimidine Tract-Binding Protein - Genetics - Metabolism |
| الوصف: | Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124 directly targets PTBP1 (PTB/hnRNP I) mRNA, which encodes a global repressor of alternative pre-mRNA splicing in nonneuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2 (nPTB/brPTB/PTBLP), an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay (NMD). During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124 plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124 promotes NS development, at least in part by regulating an intricate network of NS-specific alternative splicing. © 2007 Elsevier Inc. All rights reserved. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1097-2765 |
| العلاقة: | Molecular Cell; http://www.scopus.com/mlt/select.url?eid=2-s2.0-34547212309&selection=ref&src=s&origin=recordpageTest; Molecular Cell, 2007, v. 27 n. 3, p. 435-448; 448; 1089251; WOS:000248823400008; eid_2-s2.0-34547212309; 435; http://hdl.handle.net/10722/180725Test; 27 |
| DOI: | 10.1016/j.molcel.2007.07.015 |
| الإتاحة: | https://doi.org/10.1016/j.molcel.2007.07.015Test http://hdl.handle.net/10722/180725Test |
| رقم الانضمام: | edsbas.32DDD587 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10972765 |
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| DOI: | 10.1016/j.molcel.2007.07.015 |